Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can anyone please help me in processing SAR SLC data to generate interferogram using SNAP?

I have two scenes of the study area, one top and the other bottom. As per the tutorials and papers published, the step include coregistering the master sand slave images. However, when i try to...

24 February 2021 6,372 1 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Regarding to presentation of pairwise comparison of data?

22 January 2021 8,648 1 View

Can I use hcl to adjust ph rather than glacial acetic acid?

In alkaline lysis solution3 acetic acid should be used to adjust pH according to protocol. Can I use hcl instead of glacial acetic acid? Will there be any change in composition of solution?

08 January 2021 7,764 3 View

What is the best method for plasmid dna extraction??

I want to extract plasmid dna from bacterial culture guide me what will be the most appropriate method.

05 January 2021 1,118 14 View

How can we find crop transpiration (Tr) value from ETo values in Aquacrop model?

it like how we can get Tr value separately from ETo values in Aquacrop

20 December 2020 5,817 3 View

How to find load / force acting on the top face in ansys workbench?

The image attached is the representation of my application. This is an assembly of three items (top plate, bottom plate and the 5 load cells). The bottom plate is fixed and a load of 40 Kg is...

15 December 2020 1,145 6 View

Unlikely condition in the water inundation area after the cyclone?

I have done flood mapping with Sentinel 1 data for 3 districts of India (West Bengal) i.e. North Pargana, South pargana and East Medinipur. North and South Pargana are showing increasing trend in...

11 December 2020 4,714 3 View

How to calculate Area-Weighted Average Temperature at outlet in Ansys?

Suppose a fluid is flowing between two parallel plates (and exchanging heat) for 100-time steps. So, there is an area-weighted Average temperature at every 10 steps. How can I write a UDF to...

29 November 2020 6,240 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View