For an HPLC System, spectrum bandwidth mentioned as 8 nm. How to calculate this spectrum bandwidth?

Most of the HPLC Brochures/Specifications sheet mentions 'spectrum bandwidth' as 8 nm. But, how to calculate this one. Please suggest.

12 December 2019 4,770 3 View

Is there any Alternative for Sodium Naphthalene Sulfonate?

I am looking for alternate to SNF's (Dispersing Agent). The present one is in yellow colour and I am looking for the similar type of Dispersing agent in white colour.

29 August 2019 7,921 1 View

How to keep reference in predictor variable and outcome variable in multiple logistic regression analysis by using EPI INFO 7 Software?

HI, I am analyzing data related to a project entitled "Assessment of stigma, fear and discrimination among healthcare workers towards PLWHIV". We are planning to associate socio-demographic...

21 April 2019 1,615 2 View

Can we leverage Artificial Intelligence & Image Processing for Online Inspection of Packed Cases..?

TITLE: Leveraging Artificial Intelligence & Image Processing for Online Inspection of Packed Cases 1.BACKGROUND: Leaves of a crop are threshed and are packed into 200 Kg cases. 10% of these...

06 February 2019 8,322 2 View

Anyone using Flowing software for Flow cytometry data knows how to collect all Overlay histograms together in the Image collector?

I want to compare how the cell population is changing from time zero through the process, for which I want to see the chnage in the FSC histogram for each sample with respect to the time zero. I...

26 December 2018 2,566 3 View

How can i create a set of nodes during the analysis of model in abaqus?

22 January 2017 4,114 6 View

16S rRNA gene of bacteria is amplified but not able to sequence?

I was trying to sequence 16S rRNA gene of a bacteria. The gene is being amplified but unable to sequence it. Sequencing was attempted for 3 times but failed. What might be the reason?

27 May 2016 1,341 8 View

Does bacteria alter /adjust the pH of the liquid media itself while growing?

08 May 2016 887 15 View

Is there any Procedure to Prepare in situ preparation of CuO Nanoparticles inside PVA network.

I want to prepare CuO nanoparticles in side poly vinyl alcohol network. When ever i am trying to react copper ions in PVA solution with naOH, I am getting Green precipitate.This is copper...

07 March 2016 9,217 4 View

Fresh, decontaminated, hypo and UV treated wares and area being used as suggested. Still false positive appears in no template control, why?

I am using all the fresh working stocks, water, aerosol tips, changed the working room areas and further more decontaminated the working area with bleach and UV. Still than consistently false...

27 September 2015 2,698 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View