Your dna samples are partially degraded and some contain a lot of rna but if your next step is a pcr then I would expect them to be good enough to amplify a product.
Your samples may have been stored for too long or thawed for too long before isolating the dna.Try to minimise the time that the sample stays as a crude extract before separating the dna
Narayana Ruthwek Mamillapalli 1) Take only 2-3 plant samples (to make all protocol faster) and try 2-3 different DNA isolation methods; 2) reduce plant tissue weight per sample; 3) always use chilled tray for Eppendorf tubes.
The DNA is smeared cause of improper handling. Do the proper handling during crushing and also reduce the amount of agarose to make the gel, so that the DNA can run easily into the gel.
due to high concentration of DNA in the samples in the top row bands were observed like that I have diluted the DNA samples to 10 times and tried gel electrophoresis the results were good
I have also tried PCR with four DNA samples (Concentrated DnA one's) and the results were good there is no problem in using these samples for PCR
I have also added 2 microlitre RNase to 100 microlitre DNA samples this improved the quality of DNA
I'm adding picture results of concentrated DNA bands (Top row) and 10 times diluted DNA ( bottom row) and aslo PCR results of four DNA samples here
I would like to thank everyone for your suggestions