Performing scan electron microscopy for gill tissue and i am looking for if i can keep it for overnight or not in 2.5% glutaraldehyde.
2.5% glutaraldehyde is commonly used in our laboratory for tissue fixation for transmission electron microscopy and 24 hours is usually long enough.
You must remember however to cut the gill into little pieces (recommended ~1mm at least in one dimension) before fixation in glutaraldehyde. This compound is relatively big so it preserves ultrastructure better than paraformaldehyde, yet penetrates the tissues less effectively. If your samples are too big, fixation may be incomplete.
Also, according to Bozzola J (2014) "Electron Microscopy. Methodos and Protocols", in the chapter on specimen preparation for SEM:
"Transfer the specimen into a container of 2.5 % glutaraldehyde in phosphate buffer so that it is submerged. Fixation is normally carried out for 1–3 h at room temperature; however, specimens may be stored in this fixative indefinitely in a refrigerator, as long as evaporation is prevented"
Also I could recommend some further reading, if above mentioned information are not sufficient: https://www.leicabiosystems.com/knowledge-pathway/fixation-and-fixatives-2-factors-influencing-chemical-fixation-formaldehyde-and-glutaraldehyde/
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