Would ethylene oxide stelization inactivate RNase and DNase?

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Mohsen Navari

The reason I'm asking this question is I'm wondering if the plastic syringes are RNase-DNase-free.

Albert Batushansky

Dear Mohsen

I think it will depend on duration of sterilization, but alkylation should inhibit RNAse.

But more generally, if you ask about usage of medical syringes for molecular biology - in my experience new sterile syringes from at least BD are safe to use. Please consider this as my personal experience and not as the fact.