I am doing experiment using Antibody DNA conjugate but I noticed the DNA has many nonspecific bindings, How could I reduce them or block them? is there a specific sequence of DNA that doesn't have any binding?
You might try blocking with salmon sperm DNA . I know this is used in other applications for block non specific nucleic acid interactions
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Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...
03 March 2021 6,317 1 View
I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...
01 March 2021 736 3 View
I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...
01 March 2021 7,396 4 View
Dear All, I am doing my MS in Electronic Businesses. And I am interested in the AI field and its applications for businesses, its benefits, impacts, etc. What kind of scientific research dominate...
26 February 2021 8,816 6 View
I have a pipe with 10 mm in Diameter and 2 bar pressure, the fluid velocity is 200 m/s, the fluid is air. is there a way to reduce velocity without affecting the pressure value?
25 February 2021 7,187 3 View
I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...
23 February 2021 1,616 5 View
Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...
22 February 2021 621 1 View
We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...
22 February 2021 2,072 3 View
I am currently doing my Master Thesis in FinTech and I am thinking of doing my project on the impact of AI and ML on the financial industry but as the impact of these technologies on the industry...
21 February 2021 7,128 3 View
Please suggest ways to learn Fuzzy VIKOR methodology in a best possible way. And is there any toolkit available for the same? Thank you in advance.
20 February 2021 8,271 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...
28 February 2021 7,800 6 View
Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...
25 February 2021 9,659 2 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
The impact factor is a measure of the importance of a journal based on the number of citations received in a year. ... Articles published in a journal with a high impact factor do not all receive...
24 February 2021 7,481 2 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...
19 February 2021 7,006 5 View
How to conjugate two acids with amine linker?
18 February 2021 4,083 2 View
18 February 2021 6,665 6 View