16 Questions 8 Answers 0 Followers
Questions related from Mohamed H. Yousef
Can anybody help with delineating general tools for determining the stoichiometry of enzyme subunits in vitro and in vivo?
11 April 2021 5,506 3 View
To evaluate the anti-inflammatory effect of a number of drugs on microglia, I've used the Griess reagent to determine the levels of nitric oxide in the cell culture supernatant. Cells were...
22 February 2021 4,647 4 View
I am working with SIM-A9 murine microglia. According to the ATCC and several publications, these cells can be stimulated by LPS at a dose as low as 2.5 ng/mL. In the first round of experiments,...
20 October 2020 7,054 3 View
I am working with mouse microglial cells and the culture protocol calls for supplementing culture medium with 10% FBS and 5% horse serum (both heat-inactivated). While the FBS is abundantly...
11 July 2020 8,658 0 View
Hi all I want to assess the kinase activity of the different GSK3 isoforms in murine cells treated with inhibitors specific to these isoforms. I can only find assay kits for the GSK3B isoform....
23 October 2019 9,249 1 View
I will be working with a murine microglial cell line and intend to test the effect of a compound on the phagocytic activity of a microglia. The compound has a putative activity against a type of...
21 October 2019 7,375 0 View
I shall be assessing the migratory capacity of activated microglia in response to treatment with a number of agents. I was wondering if there are any kits that are more preferable over the wide...
21 October 2019 5,941 3 View
I am looking to isolate exosomes from a subset of T cells and re-administer them to mice. The isolation of such cells is incredibly expensive and the murine cells are not commercially available....
28 February 2019 5,373 1 View
If I am to isolate peripheral exosome for analyzing their protein content, which sample type is more advisable, plasma or serum?
08 January 2019 7,657 5 View
For my thesis work, I will be isolating exosomes from blood. However, the samples will be collected in a remote location and will not be processed immediately. What are the optimal conditions for...
08 January 2019 2,063 6 View
I need to examine the effect of an agent on neuronal cells in culture. To do so, I will be pursuing neural differentiation from fibroblasts. However, whereas a number of sources indicate the...
30 December 2018 5,560 3 View
I am looking to sort exosomes via tissue-specific surface markers, using antibodies. Fluorescence-assisted vesicle sorting be carried on the same equipment used for FACS?
01 November 2018 8,024 3 View
I am looking to detect liver-specific CD markers on exosomes. Are there any defined such markers that wouldn't be elsewhere expressed?
01 November 2018 3,680 0 View
Can antibody-conjugated sepharose beads be used for isolation of exosomes by subsequent centrifugation?
01 November 2018 4,140 2 View
Hi everyone, I and my colleagues are trying to figure out the sample size for a study that would represent the general population. We performed an ANOVA-based Power Test to ensure statistical...
20 September 2018 5,696 9 View
Do commercial kits have an advantage over conventional ultracentrifugation? Given that the sample is comprised of serum or urine rather than cells or cell-media, could UC still be effective,...
20 September 2018 2,307 2 View
