I shall be assessing the migratory capacity of activated microglia in response to treatment with a number of agents. I was wondering if there are any kits that are more preferable over the wide range of systems available.
Corning has many options: Falcon, Transwell and Corning FluoroBlok. Choosing the most appropriate is rather confusing. However, I gather that the PET membrane with a pore size of 8 um is the most commonly used parameters. However, the right type of insert eludes me.
What are your suggestions/recommendations?
Hi Mohamed. The pore size for the migration assay depends on your cell type. The following table is very useful (I took it from this website: https://cellqart.com/downloads) Good luck!
Many thanks @Karina for your helpful reply. I also found this guide by Corning, which was also quite informative.
I would not use a transwell assay to measure glioma chemotaxis. They are too full of artifacts, complications and confusions. You need an assay where you can see the cells move and ascertain what they are doing over time.
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