26 Questions 98 Answers 0 Followers
Questions related from Ming Chuan Yu
Can anyone give web links and published e-books and papers telling about why anti-tumor drugs killed cancer cells first before normal cells/stem cells? Generally it must be related to metabolic...
02 February 2018 8,206 0 View
writing 10 power 4 such as 10^4 or 10e4 rather than making 4 as super script in publish papers. Can let let me have links for such papers if you have seen. Thanks.
01 January 2018 3,146 0 View
I was looking for ingredients of serum free freezing medium but I cannot find anywhere or its discussion in this research gate. So Can anyone share your knowledege on making serum free freezing...
10 October 2017 9,220 5 View
I like to know whether it is possible just to wash out trypsin with PBS, centrifuge followed by resuspending the pellet with serum free medium for cells to be cultured with serum free medium?
09 September 2017 4,267 6 View
I would like to know your experience on culturing Human breast cancer stem cells (Parental) Thanks.
08 August 2017 7,294 4 View
I would like to get some practical info with regard to biolog PMM phenotyping. I also have done some and would like to share info too.
07 July 2017 1,237 2 View
I get fold change of miRNAs expression after running qPCR. I would like to know whether I need to do statistical analysis for those fold change or not? in fold change, if expression level is above...
06 June 2017 4,979 5 View
I am currently culturing primary breast cancer epithelial cells from human breast cancer patient. It has been about 3 to 4 months and now epithelial cells are growing well and no more fibroblast...
10 October 2016 3,026 4 View
How should we look for list of journals of Q1, Q2, Q3 respectively? I cannot find any proper list. Some said the higher the impact factor, the higher the tier is and other said no, it is not as...
08 August 2016 2,475 0 View
I would like to add one query here about plate reading wave length. It is very confusing as some use 570 and ref 630, some other use 540 and ref 720, and also found 570 but no reference. So what...
07 July 2016 831 6 View
I am facing an issue of debris after dissociation. These debris are mixed with dissociated cells. As I am using serum free and ultra low attachment flask, it is really hard to remove them. If you...
07 July 2016 8,959 2 View
I have done MTT assay, RTCA and finally determined IC50 of a compound. However, during apoptotic study, the result did not show 50% of cells were dead or apoptotic. It was less than 50% and it was...
06 June 2016 8,616 9 View
I would like to find out whether anyone can share graphpad prism software. I would like to have it. Thanks. Trial version just can be used for a month only. Thanks,
05 May 2016 314 3 View
Hi All, I found that IC50 value is not appropriate to convert in vivo dose for therapeautic or toxocity study and noted that there is no formula to convert. My stupid question...in such sense,...
04 April 2016 7,495 13 View
Can we use just normal flasks that are routinely used for cell cultures? I realized that it is the best if we don't let cells to attach the surface when culturing stem cells, especially breast...
04 April 2016 6,712 2 View
Is anyone here culturing H9C2 cells? My H9C2 cells growth rate is very slow and I am not sure what is the problem with this. I am using DMEM with 10% FBS and 1% sodium pyruvate and Passage 24 of...
12 December 2015 1,747 7 View
If you are using disinfectant into waterbath, can you let me know what is the brand and chemical name of it. Do you just use CuSO4 or bleach into waterbath? Thanks.
12 December 2015 1,483 0 View
I noticed one of my friends' cells were contaminated with active moving very small rounded black dots and also some white microbes which are a little bigger than those of black dots. These are...
11 November 2015 3,194 9 View
Hi All, I would like to check whether commercially available cryovials with silicone gasket, internal thread are safe enough to store in liquid phase of nitrogen tank or not. I see all labs are...
11 November 2015 3,881 10 View
I want to find out whether it is best to aliquot DMSO 1 ml each in eppendorf tubes and keep in -20'C so that this minimizes contamination since we used just 10% for each cryo tube. I have been...
06 June 2015 792 4 View
Because many papers only showed IC50 and one book author suggested to calculate and add to show GI50 too. Can any one suggest for this? Thanks.
01 January 1970 2,578 0 View
Nowadays, we can find kits for total RNA extraction and miRNA extraction. I would like to know whether it is good to use total RNA to run 2 steps RT-PCR for miRNA expression. I will take total RNA...
01 January 1970 7,172 11 View
Does anyone here use chitinase, beta-glucanase and chitosanase as a preventive reagent for fungal and/or yeast in cell culture? I found someone in reseachgate recommended this over fungizone.
01 January 1970 6,496 1 View
I would like to understand more and its experimental methods for Colony forming assays for cancer cell line treated with a novel compound. If you know any useful resources with regard to colony...
01 January 1970 4,885 0 View
I would like to find out how many % of FBS and what media are used when you did your MTT assays with breast cancer stem cells. Some said that they used 10% FBS with DMEM/12 and incubate in...
01 January 1970 365 6 View
I want to learn your experience with regard to cancer stem cells sorting by using column free MACS method. Thanks.
01 January 1970 6,545 2 View
