Biochemistry, Chemistry
I would like to know the answer and the solution procedure of this problem.
31 December 2019 5,506 3 View
The molecular weight of my protein sequence is 41 kDa. The SDS-PAGE result demonstrated its size 36 kDa. What are the reasons behind that?
01 February 2018 7,339 3 View
I would lie to develop some mutants.
01 February 2018 3,969 0 View
I would like to know the easy explanation for these tree points. I am happy if someone send me some articles or books about this.
01 February 2018 3,504 1 View
I got the excellent protein quantity after measuring the protein level through western blot analysis. Hence I am checking the corresponding mRNA in the qRT-PCR but failed to get proper mRNA...
10 November 2017 3,460 14 View
Gene , Molecular Biologist, Biostatics
10 November 2017 8,407 0 View
I would like to know the sodium sulfide actual state level in the solution. Biochemistry, Chemistry, Polymer chemistry, Biophysics
05 June 2017 4,835 7 View
Molecular Biology, Molecular docking, Biophysics
03 April 2017 8,190 0 View
Enzymology, Molecular biology, Biophysics
02 March 2017 8,053 3 View
Enzyme kinetics analysis, Biochemistry, Molecular Biology, Biophysics
11 December 2016 4,164 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...
22 July 2024 5,492 6 View
Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...
21 July 2024 1,632 0 View
what are the main points
10 July 2024 9,236 5 View
Hello dear colleagues and professors Please what is the recommended type of pseudopotentials for QE? ultrasoft or norm conserving?? And from where get all types of pseudos (us, nc, pbe,...
09 July 2024 9,541 5 View
