Hi,
Has anybody ever seen stained cell population moves downwards compared to FMO?
Hi, I'm analyzing some bulk RNAseq data from T cells under different conditions, and based on the RNAseq data, condition A shows higher expression of genes associated with effector functions...
01 July 2024 741 2 View
I used SD rats (E19) for cortical neuron extraction. Due to the need for subsequent special experiments, I cultured the neurons in a Corning PDL 384-well plate. The culture medium is Neurobasal +...
12 June 2024 1,714 4 View
I want to construct a nomogram for predicting the risk of fistula after esophageal surgery through comprehensive analysis of the currently published literature, and then verify its efficacy using...
25 May 2024 669 0 View
Does the catalytic activity of a lyase, such as a protease, change upon the formation of a μ-gelzyme?
21 May 2024 9,403 0 View
What is the principle of surface acrylation of enzyme protein ?
20 May 2024 9,428 0 View
Many literatures have followed Antras(2012)'Upsrteamness index of industry and combined trade data of firms to measure firms' GVC position. So, is there any better ideas or...
28 April 2024 2,607 4 View
Why papain is easily cross-linked by glutaraldehyde, while bovine serum albumin, keratinase is difficult to be cross-linked
17 April 2024 7,782 0 View
Does anyone know how to solve the magnetic structure by simulated annealing what are the specific steps, the official tutorial is not detailed, I do not know how to operate
26 March 2024 6,549 0 View
fullprof
26 March 2024 6,499 0 View
Dose and time of upregulation of CD86 and CD80 in RAW264.7 cells using LPS/IFNgamma？
24 March 2024 5,575 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,337 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,726 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,706 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,733 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,622 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,482 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,519 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,905 0 View
