Hi,
I have a general question about pathway analysis in scRNAseq. I'm under the impression that scientists pick pathways relevant to their study from the list of GO terms and present those in a manuscript, but I also read that sometimes people pick the relevant pathways only generated from either upregulated or downregulated DEGs and present these. I'm not sure which way is more accurate or reliable, or is there any specific rule to follow regarding picking the pathways?
Hi Kun
it depends on which way you want to travel, on a known but reliable one, or to be more adventurer and discover what people haven't yet published....
science is a perpetual move, with new technologies, power and discoveries, don't miss the train.
all the best
fred
interpretation is always subjective, associated to your knowledge and what is known on the subject, but try and see if you can deal with...
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