4-HT Tamoxifen concentration in primary endothelial cells?

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Beta galactosidase assay in milk?

I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...

12 June 2024 7,331 1 View

What to do if Cronbach’s alpha is unacceptable on the Big Five Inventory (BFI-10)?

Internal consistency for Neuroticism, Extraversion, and Openness to Experience, Agreeableness, and Conscientiousness subscales is unacceptable (Cronbach's alpha = 0.49, 0.50, 0.45, 0.05, 0.40)....

04 June 2024 6,224 0 View

How are DNA Ladders predetermined?

How do companies or scientists predetermine DNA ladders for Gel Electrophoresis? It makes no sense how they would know? I mean DNA is so incredibly small. As it separates in the gel how can they...

30 May 2024 1,317 0 View

Using BL21(DE3)PlysS without adding glucose?

Dear all, I wonder if it is convenient to use BL21(DE3)PlysS without adding glucose to express protein of interest? if its okay, do you have a reference ?

05 May 2024 3,465 0 View

Are there any resources on gamification methodologies not based on direct or indirect competition?

I'm attempting the development of a gamified training system, and most resources I have found on the subject so far emphasise competitive mechanics, such as scoreboards and awards as methods of...

09 April 2024 4,993 1 View

What is the RIN requirement for bulk RNAseq?

what is an acceptable RIN level for an RNA sequ experiment? G

01 April 2024 3,448 3 View

How can the future impact of the climate crisis on mental health be quantified?

The massive consequences of climate change for mental health are well documented (e.g. AR6 WGII, Chap. 7, p. 1076ff., Watts et al. 2015, Walinski et al. 2023). These will continue in the near and...

29 January 2024 5,118 3 View

Why in genetic studies do they often control for only Caucasian individuals?

Especially since the 1000 Genomes project identified possible ancestral markers.

26 January 2024 8,167 1 View

How will the overlap of Artificial Intelligence, Quantum Computing and Blockchain Technology shift the foundation of the web?

Within Artificial Intelligence there is an assumption that it is speeding things up. We are gathering and getting information organized faster than ever before. This shift in how much data and...

23 January 2024 6,357 3 View

SAP2000/ETABS : Why is my truss taking excessive values of axial force?

I modelled a 3d truss with one storey concrete structure attached to it as shown on the model. I know that the one storey concrete structure changes the rigidity and the flow of the forces on the...

02 January 2024 6,810 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View