I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a alternative with a more reasonable price?
Dear Jef,
thank you. This one I know. Unfortunately seems their is no real competitor on the market.
I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...
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04 June 2024 6,224 0 View
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30 May 2024 1,317 0 View
Dear all, I wonder if it is convenient to use BL21(DE3)PlysS without adding glucose to express protein of interest? if its okay, do you have a reference ?
05 May 2024 3,465 0 View
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09 April 2024 4,993 1 View
what is an acceptable RIN level for an RNA sequ experiment? G
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29 January 2024 5,118 3 View
Especially since the 1000 Genomes project identified possible ancestral markers.
26 January 2024 8,167 1 View
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23 January 2024 6,357 3 View
I modelled a 3d truss with one storey concrete structure attached to it as shown on the model. I know that the one storey concrete structure changes the rigidity and the flow of the forces on the...
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Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
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I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View