Did anybody try to passage mouse neonatal cardiomyocyte cultures? The cells are usually grow well. I just need to change the plate format from 6 well to 96 well for the Elisa reader. What is the best protocol/enzyme to use?
Is there a Python project where a commercial FEA (finite element analysis) package is used to generate input data for a freely available optimizer, such as scipy.optimize, pymoo, pyopt, pyoptsparse?
18 October 2020 3,310 3 View
Four cacti in our conservatory carry holoparasitic plants inside them which have flowered or are still flowering this year. They appear to be two species. Can anybody answer these questions: -...
24 September 2020 9,749 3 View
My fluent is not launching in series at all, even when I uncheck the parallel option and i also have no option for using series. Please help!!! Many thanks!
04 September 2020 4,199 4 View
If i use the MHD module, i will have to provide the values of external magnetic field, but isn't the magnetic field established as a consequence of the current flowing through the air? How am i...
15 August 2020 200 1 View
Unfortunately, we just realized after data collection, when we wanted to start analyzing our data, that some items were missing in the online survey. The survey consisted of three parts (three...
21 April 2020 6,736 11 View
Dear all, I am culturing some suspension cell lines. I am planning to perform proteomics (LC-MS/MS) experiments on them. Unlike adherent cells (viable cells are adhering to the substrate and...
03 March 2020 2,329 3 View
Dear colleagues, I would like to seek your kind advice on click chemistry enrichment of azidohomoalanine (AGA)-tagged proteins. From literature, I learned that there are 2 main avenues, either...
18 February 2020 8,725 2 View
I am looking to find contemporary (2005 and onward) LGBTQ+ literature/texts (novels, poems, digital literature, correspondence, social media writers) by Ecuadorian writers (preferably LGBTQ+ too,...
04 February 2020 9,780 3 View
It has been found feeding on Eucalyptus leaves in Indonesia. Family or genus, maybe? Thank you.
25 December 2019 6,971 3 View
I have been screening the activity of immobilized enzymes in shaken tubes and need to move into testing activity under flow in a column. I am looking for recommendations on what column / pump /...
22 November 2019 6,444 1 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View