The MW of VEGF-C I'm considering to use is 15.5 kDa from R&D. The VEGF-C is released from PEGDA. How can the coefficent be calculated so one can control the release rate?
If you are willing to assume that VEGF is approximately a spherical protein, then you could calculate its molecular radius and use the Stokes-Einstein relation to calculate D. There are probably other issues involving the equivalent viscosity of the PEGDA hydrogel, but this would still be a good place to start.
Yes, it would be a good start to calculate it using Stokes Einstein equation or others such as Wilke Change. You would probably also have to assume that there is no molecular interactions (which may not be true for large MW substances). However, I think the critical issue is to know if the molecule (VEGF) is released into a liquid (e.g., cell culture medium) or a tissue or some sort of membranes or scaffold? The source of VEGF is less important as it does not effect diffusivity. Depending on the medium VEGF is released, it may be worth doing a simple set of experiments to determine. I have listed three of our papers which you may have a look..
Suhaimi, H, Wang, S, Thornton, T, Das, DB (2015) On glucose diffusivity of tissue engineering membranes and scaffolds, Chemical Engineering Science, 126, pp.244-256, ISSN: 0009-2509. DOI: 10.1016/j.ces.2014.12.029.
Suhaimi, H, Wang, S, Das, DB (2015) Glucose diffusivity in cell culture medium, Chemical Engineering Journal, 269(June), pp.323-327, DOI: 10.1016/j.cej.2015.01.130.
Han, T and Das, DB (2013) Permeability enhancement for transdermal delivery of large molecule using low-frequency sonophoresis combined with microneedles, Journal of Pharmaceutical Sciences, 102(10), pp.3614-3622, ISSN: 0022-3549. DOI: 10.1002/jps.23662.
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