I'm looking at injecting VEGF-C into a rat model, specifically into the medial right thigh tissue. I'm going to do this after removing a lymph node.
From the literature i have seen so far, most researcher's have combined VEGF-C with a hydrogel (HAMC [Baker et al., 2010] or Gelatin [Hwang et al., 2011]) before injecting into the lymph node region.
Can VEGF-C brought stragiht from a manufacture (eg R &D - Recombinant Human VEGF‑C) be injetcted into rat tissue without further modification? if modification is needed, can VEGF-C be dissolved in PBS prior to injection?
NB: Dont wont want to use Adenovirus VEGF-C.
I'm asking this question because time is short to prepeare a injectable delivery vehicle for VEGF-C. However i'am open to suggestions which are simple and can be manufactured in a hour.
The volume of VEGF-C i want to deliver are similar to the values mentioned by [Baker et al., 2010] and [Hwang et al., 2011
Thank you.