I'm looking at injecting VEGF-C into a rat model, specifically into the medial right thigh tissue. I'm going to do this after removing a lymph node.
From the literature i have seen so far, most researcher's have combined VEGF-C with a hydrogel (HAMC [Baker et al., 2010] or Gelatin [Hwang et al., 2011]) before injecting into the lymph node region.
Can VEGF-C brought stragiht from a manufacture (eg R &D - Recombinant Human VEGF‑C) be injetcted into rat tissue without further modification? if modification is needed, can VEGF-C be dissolved in PBS prior to injection?
NB: Dont wont want to use Adenovirus VEGF-C.
I'm asking this question because time is short to prepeare a injectable delivery vehicle for VEGF-C. However i'am open to suggestions which are simple and can be manufactured in a hour.
The volume of VEGF-C i want to deliver are similar to the values mentioned by [Baker et al., 2010] and [Hwang et al., 2011
Thank you.
The only reason they used Hydrogel is to increase the solubility or to localized the VEGFC into tissue only. If VEGFC is soluble in PBS , u can inject it directly.
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