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I am in the process of making graphs for my scientific data and I want to know what is the best software available and most easy to use that you already are familiar with.
19 September 2023 8,240 5 View
Hello dear, I would like to do a fatigue experiment on the Ansys program for a particular human bone. I need S-N curve where can I find it away from practical experiences
31 July 2020 2,553 5 View
Hello, Is it necessary to change culture medium before transfection. My cells abruptly lose attachment upon medium change. Thanks.
01 June 2020 4,035 4 View
Hello Is there any optimal condition of centrifugation (speed, time) to obtain cell pellet in a small 1.5mL tube. What I am looking for is a method of harvesting living cells and floating cells...
31 May 2020 5,046 0 View
The role of chaetomium globosum in controlling plant diseases
22 May 2020 8,656 3 View
Can the technical integration ability of pre-service teachers be improved by STEM? If it is a multicultural area, can it highlight the cultural characteristics of the region to improve the...
17 October 2019 164 1 View
I need to figure out a way how to modify the graphs. By modifiying the graphs I mean changing the frame or the style that makes the line of each graph, also, I want to change the filling of each...
13 August 2019 8,605 2 View
Hello, I want to study the effect of EGF on the expression of a target protein. Is there any alternative to serum starvation? Do you think that treating cells with a high concentration of EGF in...
25 December 2018 8,948 3 View
silver nano
21 July 2016 8,976 3 View
Hello everyone, I am really intrested on the mechanisms of virus entry and replication of HBV and HCV in different hepatoma cell lines. I heard about the new established hepatoma cell line,...
11 May 2016 4,272 1 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...
08 July 2024 1,330 0 View
Looking for a protocol for the stimulation of B cells with pokeween mitogen
22 June 2024 4,882 4 View
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View
I am working on a project that requires stimulating RAW 264.7 NF-kB macrophage and readout my signal by luciferase assay. Although all concentrations tested (10, 50, 100ng/mL) after 24 hours...
16 June 2024 2,885 3 View
I use raw 264.7 NF-kB cell line and the empty wells has a high absorbance as close as the blank well.
20 May 2024 1,518 4 View
Hi All, Our group is doing some flow cytometry assays using an HLA-A*0201 tetramer presenting an HBV peptide (FLLTRILTI) and we're getting some strange results... We ran tetramer flow on a bunch...
16 May 2024 8,123 1 View
Are there any specific considerations for the dosing and administration of dexmedetomidine in patients with renal or hepatic impairment?Dexmedetomidine: A Comprehensive Review of Pharmacology,...
08 May 2024 1,747 1 View
Based on the article by Kavulavu et al. (2022) about the challenges facing blood transfusion services being faced by Kenya during blood transfusion states, one of the problems faced is that the...
11 April 2024 1,492 2 View
