fatigue failure S-N curve ?

Hello dear, I would like to do a fatigue experiment on the Ansys program for a particular human bone. I need S-N curve where can I find it away from practical experiences

31 July 2020 2,340 5 View

Medium change before transfection?

Hello, Is it necessary to change culture medium before transfection. My cells abruptly lose attachment upon medium change. Thanks.

01 June 2020 3,811 4 View

Does chaetomiun globosum have a direct effect on pathogenic fungi, or is it limited to stimulating plants to resist?

The role of chaetomium globosum in controlling plant diseases

22 May 2020 8,510 3 View

How i can calculate nano particles concentration from their OD , when their weight is unkwon?

silver nano

21 July 2016 8,846 3 View

Does the hepatoma cell line, HLCZ01, really support HBV and HCV entire life cycle?

Hello everyone, I am really intrested on the mechanisms of virus entry and replication of HBV and HCV in different hepatoma cell lines. I heard about the new established hepatoma cell line,...

11 May 2016 4,126 1 View

HBx detection via Western Blot?

Hello everyone, I am trying to detect HBx protein from Hepatitis B Virus through Western Blot. Knowing that the molecluar size of this protein is 17kD can anyone give me an indication about the...

26 April 2016 6,580 2 View

Core and HBx proteins western blot detection?

17 April 2016 6,828 4 View

Is the permeabilization step essential in Rhodamine 123 mitochondria staining?

11 April 2016 7,012 4 View

Hbx snd core protein western blot detection?

Hello everyone, I am trying to detect hbx and core protein with western blot, is it better to use a 10% or 12% Sds-polyacrylamide gel? Thank you in advance

04 April 2016 2,948 3 View

Rhodamine 123 staining protocol for Flow cytometry?

01 April 2016 5,451 5 View

Do you have any experience with transfection of EBV immortalized B cells with Lipofectamine?

I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...

05 February 2021 4,159 1 View

Does euthanasia by CO2 inhalation cause NF-kB activation in mouse heart or body in general?

I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?

26 January 2021 4,096 2 View

Can fibrosing cholestatic hepatitis in an immunocompromised patient be diagnosed without a liver biopsy?

provided that all the criteria for diagnosis has been fulfilled and alternative diagnosis are also ruled out. The patient has coagulopathy and transjugular biopsy wont be possible due to resource...

13 January 2021 8,935 4 View

Can anyone recommend utilizing the plasmid extraction kit for extraction of the circular DNA genome of viruses associated with insect vectors?

Dear Colleagues, I am planning to detect and discover DNA viruses particularly those with a circular genome and transmitted by insect vectors. However, I am confused about selecting the best...

10 January 2021 9,534 4 View

You can mention a contribution that science will achieve in this year 2021?

It is known that scientific knowledge is replicated each time at more unusual speeds, however, humanity expects more from our scientific work, that is, solutions to problems that have accumulated...

02 January 2021 8,300 16 View

Why can I not see any plaques when performing a viral plaque assay with MHV?

28 December 2020 5,515 2 View

What can be used as a positive control for Annexin V/ 7AAD stain with BL6 mice STAT3 Knock Out B-cells?

I am trying to isolate B-cells from the spleen of a STAT3 KO mouse and plan to stain with Annexin V and 7-AAD. I will then use flow to determine apoptosis and overall cell death. I have used...

18 December 2020 9,600 3 View

Why is SV40 not considered an oncogenic virus?

Hello, SV40 is not considered an oncogenic virus in this review:Oncogenic viruses and cancer Why is SV40 not considered an oncogenic virus when it produces the large T antigen, which is...

04 December 2020 8,374 5 View

B12 deficiency resistant to parenteral treatment ?

hello i have a 40 year old female patient with chronic fatigue and forgetfullnes who also has hashimatos disease wich is under control. Her serum B12 level vas 164 pg/ml. After treatment with a...

02 December 2020 6,676 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View