Dear Gangula Abilash

Nitrocellulose membrane is commonly used for nucleic acid and protein detection in research and diagnostic applications. The high protein binding capacity is ensured due to the homogenous nature of the membrane. Other nitrocellulose membranes contain high levels of cellulose acetate, which reduce the protein binding capacity of the membrane. Physical characteristics of the nitrocellulose membrane, such as high tensile strength and hydrophilicity, provide excellent handling ensuring that the membrane will not rip, tear, or crack during transfers. The detergent free and unsupported nature of this material yields sharper images with little or no distortion. Compatible with a variety of detection systems, it has lower protein burn through than competitive nitrocellulose media in electrophoretic transfers. Highly consistent, both inter- and intra-lot, this membrane will provide sensitive detection of biomolecules blot after blot.

Pls. find the attached files may  you get the right answer

http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/GELifeSciences/products/AlternativeProductStructure_16756/

http://www.sigmaaldrich.com/labware/labware-products.html?TablePage=17215790

http://info.gbiosciences.com/blog/bid/203026/PVDF-or-Nitrocellulose-Which-Membrane-is-Best

https://www.researchgate.net/post/Why_protein_from_SDS_PAGE_is_not_transferred_onto_nitrocellulose_membrane

http://www.ncbi.nlm.nih.gov/pubmed/1789412

http://www.ncbi.nlm.nih.gov/pubmed/2438293

Regards

Prof. Houda Kawas

I think of the membrane as a fairly flat surface with many binding sites for proteins or nucleic acids. One normally transfers the proteins or nucleic acids onto the pristine membrane, taking up some of the binding sites but leaving others unoccupied. These remaining sites must be blocked in order to prevent the probe (antibodies or nucleic acid) used to detect the transferred target proteins/nucleic acids from sticking to the surface of the membrane. For Western blots, one usually uses BSA or the proteins in nonfat dry milk to block the membrane.  There are also protein-free blocking agents. After blocking, there are no more free sites left on the membrane. Now when the probe is added, it can stick to the target but not the membrane. The blocking agent will not normally stick to the immobilized target.

Here is a discussion about nitrocellulose membrane in this context:

http://what-when-how.com/molecular-biology/nitrocellulose-molecular-biology/