Fayaz Ahmad Mir

11 Questions 10 Answers 0 Followers

Questions related from Fayaz Ahmad Mir

What are the easiest ways to measure ROS in a specific brain area in free floating brain sections? How stable are the ROS products after perfusion?

I would like to measure ROS by measuring a good indicator of it such as lipid or protein based peroxidation markers etc. Do i need to perfuse he animal with PFA or saline, does PFA fixation affect...

05 September 2023 2,170 0 View

What are the easiest and quick ways to analyze EEG data into FFT, ERSP and Time-Frequency images?

Time frequency images and curves sorted by power or dB versus time are the main constituents of the EEG information. Therefore, video tutorials, links, manuals or paper-based suggestions as well...

09 February 2023 9,471 2 View

Which part of the NTS in rat does contain more chemosensory neurons?

I want to target chemosensory neurons of NTS (Nucleus solitary tractus) of wistar rat and the coordinates I have selected are AP -13.5 to 14.7 mm (From Bregma), ML -0.8 mm from midline and DV 7.8...

01 April 2019 4,580 0 View

What is the appropriate dose of Barium chloride to be injected in Rat brain to inhibit inwardly rectifying potassium channels (Kir) ?

I want to inhibit inwardly rectifying potassium (Kir) channels by barium chloride in rat brain via cannula assisted drug infusion. However most of the authors have used it in patch clamp studies...

21 November 2017 8,546 4 View

At what concentration/dose should SKF-96365 be injected in rat brain to inhibit TRPC channels in any specific brain area?

I need to inject SKF-96365 (1-[-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenethyl]-1H-imidazole hydrochloride) , an inhibitor of TRPC channels, in rat brain stem by cannula injections, but i have...

13 January 2016 7,594 4 View

I am trying to record Genioglossal muscle EMG in rats using Somnologica , ponemah and spike 2 acquisition systems. Is it feasible?

I need to filter the Genioglossal Muscle EMG at 100-1000 Hz, that is what the papers suggest. Since i am using somnologica, spike 2 and Ponemah acquisition system, i do not find such options...

10 November 2015 6,474 3 View

Double labeling for IHC on brain sections and choice of secondary antibodies?

I want to do a double labeling Immuno-histochemistry co-localization experiment for c-fos and TH in free floating rat brain sections. both the primary antibodies are raised in rabbit. Apart from...

28 September 2015 9,956 11 View

How do you measure the concentration of bicarbonate in blood samples (from animal model veins) by biochemical manual methods, not by gas analyser?

Metabolic acidosis causes variations in pH levels in blood and metabolic rate varies during sleep which in turn can result variations in CO2 levels being expelled out owing to the differential...

12 November 2014 8,560 1 View

What is the structural and functional difference between vacuolar-type ATPase present in eukaryotic cells and gastric hydrogen potassium ATPase in stomach?

V-type ATPase is the enzyme responsible for acidifying intracellular organelles like lysosomes, endosomes and synaptic vesicles. It translocates protons with concomitant hydrolysis of ATP. My...

09 May 2013 5,067 0 View

How does the level of carbon dioxide fluctuate in REM sleep? How does it affect the firing pattern of locus coeruleus neurons?

Many physiological processes like temperature, BMR, respiration rate changes in sleep wake transitions. As sleep has been categorized in to two types as REM sleep and NREM sleep, various studies...

06 February 2013 758 3 View

How can I monitor in-vivo changes in the concentration of norepinephrine in rat brain whilst the animal's sleep is being recorded by EEG?

During wake/sleep transitions in animal/birds, various circuitry in the brain undergoes change/modification, citing the role of various types of neurotransmitters in the regulation of sleep-wake...

05 February 2013 9,923 7 View