What the best protocol for protein extraction from human serum?

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Vincent Paul Schmitz

Is there a particular molecular weight range you're looking for? This can be accomplished with dialysis and there are a range of pore sizes (ex: 10 kDa) that allow you to retain only larger proteins. Size-exclusion chromatography using Sepharose or similar columns will also suffice as the largest proteins will elute first.

Fawaz Al-Shaheri

Dear Vincent Schmitz,

Thank you for your answer.

Actually I do not have a particular molecular weight. I just want to extract the whole proteins contents of human serum.

Pranita Kamble Waghmare

Various methods are available, but most conventionally performed are salting out and serum electrophoresis. For whole serum exploration I will recommend eletrophoresis. If you want to study protein with defined molecular size then dialysis, size exclusion chromatography are available. Specific protein, then affinity chromatography are routinely done.