How to mine the TCGA Database for RNA sequencing?

Anyone with advice on how to mine data from TCGA Database. I am interested in RNA seq data, i have a gene of interest i to analyze but i cannot seem to locate where to indicate the gene i am...

10 November 2019 2,362 3 View

Does any one have Experience of PEI transfection with Gastric cancer cell-lines?

Hello, I am working on gastric cancer cell line, i try to transfected the gastric cell lines (SGC-7901, AGS, BGC823) according to standard protocol, but unfortunately they kill my cells, does...

04 May 2019 1,816 4 View

What concentration of G418 (geneticin) and Puromycin you would recommended for Gastric cancer cell line?

I am using gastric cancer cell line for my research, i used Lipofectamine 2000 for transfection, could any one recommend me the good concentration of G418 and Puromycin? regards

08 September 2018 1,107 5 View

Suggest a relevent mouse model for HepG2 cell line?

suggest a relevent mouse model for HepG2 cell line, i am studying gene expression in tumorgensis. our desire gene show engough results in invitro studies, now i want to know about gene function in...

10 November 2017 4,867 6 View

Why so cell detach from polystyrene plate after 24 hours?

I culture oral cancer cells in polystyrene plate, these cell were okay up to 24 hours, after wound has been created to analysed the migration efficiency, after 48 hours when i check again these...

05 June 2015 7,416 7 View

Can any one suggest me some none-paid journals for Microbiology and Plant sciences?

can any one suggest me some none-paid journals for Microbiology and Plant sciences. No worries if journal don't have impact factor or low impact factor but it should be ISI and valid. Regards

31 December 2014 4,427 26 View

Can anyone help with the problem I have been facing in FaDU cell attachment?

hello, I culture FaDU ( oral cancer cell) for few time but i facing problem with their confluence and attachment, even i used standard procedure for culturing? i use DMEM with 10% FBS and desire...

11 December 2014 6,286 6 View

Why there are no clear bands when I run a Western blot for the GADD45a gene?

09 October 2014 4,654 12 View

How can I design an experiment for cancer treatment through Hydroxycinnamaldehyde?

I want to design the experiment for cancer (except breast cancer, colon, prostate) treatment with anti tumor agent Hydroxycinnamaldehyde with molecular mechanisms or pathway.

04 May 2014 1,080 6 View

Could you please suggest a method for metabolites profiling of plant extract through GC MS?

Could someone please suggest a method for metabolites profiling of plant extract through GCMS (aglient 5975c series)? Also, which part of the column would be suitable for plant extract?

09 October 2013 5,316 5 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View