Hello can any one please give the the BioXM software ?

i need this software in english version?

07 August 2016 4,204 2 View

Which restriction enzyme is best to use as i want to use pCAMBIA 3301 for expression analysis of a gene?

07 August 2016 9,672 3 View

Over-expression of a gene?

hello research gate. 1-how to study the over-expression of a gene ? 2-like in general, what things we should have to consider for studying the function of gene by over-expression? 3-which is a...

05 June 2016 5,363 1 View

Can any one please tell me the difference between nucleic acid and amino acids?

Hello, can i ask a question that can any one explain me the difference between nucleic acids and amino acids, in the sense of which one is lighter or heavier,? next is then which one is easier to...

05 June 2016 369 0 View

How To find the family of a gene in soybean?

I have one gene and I want to know the family of that gene and is that possible to know the function of gene by using any of twee

05 June 2016 3,019 2 View

Using pcr product and do the pcr again ?

hello, can you please tell me if i use the pcr product as a template and do the pcr again so is there any mutation will occur in my 2nd pcr product but as i check the re pcr products by using the...

05 June 2016 1,004 5 View

Soybean mosaic virus use for transformation?

05 June 2016 1,820 1 View

How to design the primer from the CDS sequence?

how to design the primer from only the CDS sequence as i want to clone my gene into the expression vector?

05 June 2016 1,751 3 View

Concentration of PCR product?

Hello, can any body please tell me how to improve the concentration of PCR product?or how to increase the concentrations of gel purified/gel extracted product? my preference is to improve the...

05 June 2016 8,484 2 View

Is there any way to check the function of candidate gene by using any software?

i just want to know the predicated function of my gene can i do this by using any software?

05 June 2016 2,240 0 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View