Can someone help me to how to optimize the chromatin digestion for my CHIP experiment?

Hi everybody, I am doing CHIP experiment. The problem is that in my gel agarose I see alot of DNA with big size (longer that 1000 bp) which means there is alot of unfragmented DNA in my gel. I...

06 September 2020 1,507 5 View

Can someone explane the difference between spheroids generated from primary cells and spheroid produced from cell line?

Hello everyone, I see a lot of papers with the spheroid generation topic. I know spheroids are used widely for simulation of tumor condition in body. Can someone say which abilities does the...

31 August 2020 3,323 3 View

Does anybody know the difference of the effect of the dextran coated charcoal stripped serum (DCSS) containing medium and DCSS containg medium?

Hi everybody, I have cultivated the C4-2 cell line in double charcoal stripped serum containing medium. A lot of the cells do not attach to the bottom of the flask. If someone has experience...

24 August 2020 9,634 2 View

Does someone have experience with cultivation of C4-2 cells in 15 cm dishes?

I am culturing C4-2 cells in 15 cm dishes in different serumes of 5% FCS and 5% DCSS. since the cells are slow in attaching to the dish in DCSS compare to the FCS if someone has experience, can...

20 August 2020 1,535 1 View

Can someone explain how we can Ioptimize antibody concentration which I wanna use in CHIP-qpcr?

Hi everybody, I wanna use an antibody to determine the presence of a protein in my chip-seq experiment. Can someone help me how can do concentration optimization for antibody which I use ? Thanks...

19 July 2020 9,619 3 View

What will the future of sustainable production and consumption within non-durable goods industry?

Assuming that non-durable goods last less than three years on average, which direction do you think the world businesses and consumers are moving considering the huge environmental impact of...

27 May 2020 4,062 4 View

What are the most influential factors that can move world businesses towards practising environmental sustainability?

The industrial world is confronting the environmental side of sustainability. But, how do you evaluate the incentives - either from society or government- to convince them about sustainability...

09 May 2020 557 47 View

Has someone experience about getting different lifetime result by changing magnification of a sample?

Hi everybody, Does anybody have experiences like getting different lifetime result by changing magnification from a sample? Can this factor lonely has influence on the lifetime of a tissue? Bests

20 March 2020 8,745 3 View

Can someone suggest a sterilized, clean and trustable pig serum company for ordering?

Hi everyone, I currently did some experiments with a pig serum (notnheat inactivated), which the control negative of that showed bacterial contamination. As I have to filter it befor using and I...

01 December 2019 8,649 3 View

How much is the possible maximum dose for cells without showing toxic effect?

I extract the primary RPE cells from pig. As I see in several time extraction high contamination of bacterial in my cell culture flask in the 3rd day after extraction. Sometimes they survive...

18 November 2019 8,623 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View