29 Questions 44 Answers 0 Followers
Questions related from Elham Amini. E.A
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
19 July 2024 1,513 2 View
Hello All, I'm doing RNA extraction from cells. I tried both ways by using TRIZOL (with chloroform, and isopropanol) and RNeasy mini kit. When I use TRIZOL, the RNA concentration is high, however...
12 March 2024 9,030 8 View
Hello All I prepared my library to run for sequencing. I would like to check the quality of my samples through D1000 ScreenTape Assay before add to sequencer. Can anyone help me to interpret my...
11 March 2024 8,717 0 View
Hello all I'm working with SHSY5Y cells to differentiate them to neuronal cells. I'm following the protocol with using RA, BDNF and cAMP for cell differentiation. I tried it different times but...
20 February 2024 1,646 5 View
Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...
20 February 2024 9,249 2 View
Hello All, I have shRNA lentivirus particles and I want to produce self-inactivating replication incompetent viral particles in cell by myself. My purpose is to produce knock-down cells in flask...
11 January 2024 6,366 0 View
Hello all, I'm doing RIP sequencing, that is RNA Immunoprecipitation followed by RNA sequencing and need help for data analysis. I would really appreciate it if you could share information,...
11 September 2023 1,215 0 View
Hi all, I would like to do Dio staining in non-differentiate and differentiae SHSY5Y cells. I really appreciate if you could share Dio staining protocol with me.
07 September 2023 9,403 7 View
Hello all I'm working with SHSY5Y cells and would like to differentiate them to neuronal cells. I used differentiate media with Retinoic Acid (RA) for this. After finishing the incubation...
21 August 2023 3,574 4 View
Hello All, I'm working on SHSY5Y cells differentiation. My problems are: 1-After adding the final differentiation media to the cell cultures, they started to detach from the plates, and became...
29 June 2023 5,807 3 View
Hello all I would like to start working with human iPSC cells, and looking for protocols that I can use it for thawing, freezing, regular passaging, and differentiate of this cell line to neuronal...
16 May 2023 7,738 2 View
Hello all I would like to start working with human iPSC cells, and looking for protocols that I can use it for thawing, freezing, regular passaging, and differentiate of this cell line. Thank s...
14 May 2023 4,911 0 View
Hi all I'm looking to buy packages that are useful for postdoc fellow in the field of neuroscience who is working in RNA sequencing as well. I really appreciate if you could suggest me any options
03 May 2023 1,521 3 View
Hi all, I'm doing immunocytochemistry to detect and visualize my protein of interest in SH-Sy5y cells, however I faced two problems: First, growing of SH-Sy5y cells on glass coverslips, which...
24 April 2023 7,843 15 View
Hello all, I used my neuronal cells (SHSY-5Y) for doing ICC. However, they washed out during washing steps. I used Poly-D-Lysin to coated all coverslips. Should I increase the concentration of...
28 March 2023 5,206 0 View
Hi, I going to do siRNA treatment in my cells with Lentivirus using MISSION® Lentiviral Transduction Particles (Sigma, SHCLNV). Has anyone use this kit before? I have some questions related to...
01 December 2022 8,263 0 View
I want to check the role of one protein in neuronal cell line. For this, I'm going to use siRNA knock down of gene for that protein, in order to check whether knock down gene can effect on cells...
22 November 2022 3,885 2 View
Hi everyone, I've already thawed my cells (SH-SY5Y) and started to seed them into flasks with using fresh media by adding 1% Amphotericin B that is antifungal, to the culture media. But,...
01 November 2022 2,824 0 View
Hello all, I am trying to isolate RNA from neuronal cells using Trizol ,chloroform, isopropanol. After reading the OD, the concentration of RNA is good, but their quality is not good. So, I...
24 October 2022 7,388 6 View
Hi all, I am facing some practical issues in subculturing of the SH-SY5Y human Neuroblastoma cell line. I've already followed the protocol related to differentiation of the SH-SY5Y human...
02 September 2022 5,939 0 View
I got similar results for acute and chronic treatments which applied in different doses. How this similarity can be explained?!
02 March 2018 9,127 6 View
The cracks exist on the surface of paraffin blocks and due to this issue, all sections which I get are cut from those parts. Please guide me, thanks.
07 April 2014 9,891 17 View
Hi everyone, I want to do PI and DAPI dual staining for my cell culture. Can anyone give me a protocol for this. Thanks in advance.
01 January 1970 8,107 3 View
01 January 1970 646 0 View
01 January 1970 6,986 2 View
Hello all, I used my neuronal cells (SHSY-5Y) for doing ICC. However, they washed out during washing steps. I used Poly-D-Lysin to coated all coverslips. Should I increase the concentration...
01 January 1970 9,096 0 View
01 January 1970 546 2 View
Hello all I'm seeking a tutorial for the new version of RIP-Seq/ChIP-Seq data analysis using Partek Flow. The YouTube tutorials are outdated, so I'd appreciate any up-to-date resources you can...
01 January 1970 8,751 2 View
Hello all I'm doing RNA seq, and using AMPure XP beads for cleanup steps and size selection of my RNA lib. Before using beads, my RNA concentration is good, however, after using Ampure beads the...
01 January 1970 8,984 2 View
