Hi everyone,
I've already thawed my cells (SH-SY5Y) and started to seed them into flasks with using fresh media by adding 1% Amphotericin B that is antifungal, to the culture media. But, unfortunately cells not attached to the flasks and all were floating. Is adding of antifungal (Fungizone) or Pen/Strep can affect to the attachment of cells?
Thanks