30 Questions 106 Answers 0 Followers
Questions related from Eduardo Goncalves Paterson Fox
I would like to publish one huge table as Supplementary resources to a forthcoming paper. The R package 'plotly' enables the creation of interactive tables which can be searched, filtered for...
09 September 2018 8,459 8 View
[Please mind that ggplot will not produce tables] I am a novice R user. I have a huge table with contigs annotations which I'd like to present in full as supplementary results. I wanted to make it...
07 July 2018 8,429 3 View
Dear colleagues, We have a Nanodrop model (Nano-300 AllSheng) which allows for scanning samples from 200-800 nm. I was thinking whether it could be used as an alternative to more sophisticate...
01 January 2018 8,122 4 View
I have been separate piperidine alkaloid isomers with ordinary normal phase silica TLC, revealed with Draggendorf's reagent. However now I am attempting some separations using a silver-nitrate...
11 November 2017 5,009 4 View
Given so many metrics available for evaluating the best journals to which one should submit one's research manuscript, I usually find the Acceptance Rate one of the most desirable. Also as to...
02 February 2017 7,027 12 View
I have searched on the website and did not find a link. Someone dropped that question but Mr Beall answered something else. Having the list offline is useful especially when discussing with my...
11 November 2016 3,809 6 View
Dear collagues, This is probably a very naive question but to date I haven't obtained a satisfactory answer. I often must study old papers. I recurrently find these old papers very reticent and...
09 September 2016 2,658 8 View
Hello, Recently I have been faced with an unexpected issue. I bought this DNA/RNA staining reagent Syto-11 which comes in DMSO. I could see the whole solution was solidified near the tube cap,...
05 May 2015 9,776 6 View
I have seen this video from BBC "Fire Ants and Techno-Chaos" which states that fire ants (mainly Solenopsis invicta) will attack electric equipments in the US, causing considerable damage and...
09 September 2014 4,794 4 View
I would need to do some experiments on ant pupae, but I should genotype them before procedures. As such I would be needing some body part for DNA extraction. I know adults do well after one leg...
06 June 2014 5,747 6 View
I am having difficulties in delivering controlled doses of dsRNA to insect pupae. Following some published protocol, I have been attempting to inject fire ant pupae with water and saline (as...
06 June 2014 2,471 22 View
I have been analysing compounds with a GCMS for over few years. Some of them I would like to test for biological activity. I have attempted liquid separation by several means with no success to...
06 June 2014 8,426 5 View
This is a weird question on a taboo topic. I have been discussing some issues about data manipulation with colleagues. Some of them believe that considerable manipulation is done in statistical...
04 April 2014 8,848 73 View
Will try to make this question as simple as possible. If anyone has any ideas, please. I am trying to silence a gene in an ant. My target gene for silencing has several spliced transcript forms,...
03 March 2014 2,292 23 View
I am not very experienced with molecular biology, and recently my advisor posed me a difficult question. I will explain as follows. I am comparing relative expression between different genes using...
02 February 2014 8,030 10 View
I am new to HPLC, and I am trying to devise a protocol to deal with poorly studied alkaloids (solenopsins). There is a variety of solvents which can dissolve these compounds, and I am trying to...
02 February 2013 3,213 21 View
I am a user of a Thermo Scientific Surveyor HPLC system with an autosampler. Since we came back from end-of-year festivities vacation, the system is not working anymore, because of unexpected...
01 January 2013 9,479 19 View
I have been having serious issues with dead volume in our HPLC system, basicallly because the tubings provided for our FRC-10A system are rather thick. I am considering changing it but it seems to...
12 December 2012 6,453 8 View
There is intense recent debate over scientific good practices, especially in the making of manuscripts and narrative and data preparation. Open discussion blogs like the Retraction Watch have made...
11 November 2012 9,278 5 View
Could anyone indicate the best (or most used) software to map stripped ESTs to a predicted transcriptome (or even predicted proteins) from a model organism genome? I would like to build contigs...
11 November 2012 6,076 6 View
I have been having difficulties with a lag between my fraction collector and the cell detector of my Shimadzu HPLC system. I am trying to adjust this delay time by determining the exact time...
11 November 2012 2,229 26 View
I have recently acquired my first Shimadzu HPLC system, and I have been trying to put it to use immediately. When recovering a sample using our fraction collector, I have noticed a considerable...
11 November 2012 6,803 13 View
I have been reading about cell-free protein expression systems. However my colleagues say they do not find many using the commercial PURE system which used recombinant expression elements. Does...
07 July 2012 1,656 0 View
Decrease in absorption at 215nm during peptide chromatography with C18. I have injected a peptide-rich mixture in a C18 column with a Thermo-Scie HPLC system. I have used a 10-80% ACN 0.1% TFA...
07 July 2012 9,222 19 View
I am rather frightened of using my brand new C18 column to test alkaloids, for fear that they would adsorb to the resine. Yet I have read protocols in catalogues and papers using acid mobile phase...
07 July 2012 288 4 View
I have been cleaning my Hamilton syringe with the cleaning wire provided. It is now bent and does not fit properly back into the case. My attempts of straightening it back up only made it more...
07 July 2012 3,871 3 View
We have been testing the effects of natural solenopsins on different fungi of economical interest. Now our sponsors want to see the results with synthetic compounds. Does anyone know where we...
10 October 2011 2,061 0 View
I have now started working with the antibacterial and toxic effects of piperididinic alkaloids. Problem is, we know very few people in our institution that is experienced with such...
08 August 2011 2,084 0 View
08 August 2011 5,382 4 View
I have just started exploring Reddit, and I believe it holds great potential for broadcasting research results and published papers. However, it is not simple to use and there sheer amount of...
01 January 1970 2,396 3 View
