hi
Look this link and PDF may help you
iwww.ncbi.nlm.nih.gov/.../PMC22461...
National Center for Biotechnology Information
by F Seki - 2003 - Cited by 147 - Related articles
Canine distemper virus (CDV) is a member of the Morbillivirus genus in the family ... Field isolates of CDV are also reported to replicate in dog and ferret ...
Good luck
yes if you are at thev iremic phase before the antibodies synthesis
later PCR is more useful
I think Pierre is right- the success of isolation is very dependent on the viraemic phase. Previous day -you could prepare some Vero cells in a small flask with 50-60% confluency. Then inoculate the monolayer with blood/plasma with sufficient amount of serum free media/sterile PBS to cover the monolayer- incubate at 37 degree incubator with 5% CO2. Shake the flask every 10 minutes - after 2-3 hrs wash the monolayer at least twice with PBS followed by addition of fresh media with 2-5% serum. You'd probably start seeing some CPE 4-5 days later if there is live virus in your inoculum.
Let me know if you require any other information. Good luck with the isolation
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