Can filter dengue virus in 0.22 micron?

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Medium replacement interfering with cell culture blot results?

We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....

17 June 2024 1,649 0 View

I have a simple question: can i use "displacement" instead of "shift" when i refer to XRD peaks?

In my new research, about TiAlN thin films, i constantly use the word "shift". I would like to know if i can write "displacement" as a synonym, with the intention to not repeat the words.

02 June 2024 9,694 4 View

Z-axis alignment in a protein for z-axis constraints in Amber software?

Good morning, I am running some Steered molecular dynamics in Amber 22 and want to set some restraints in the z-axis so as to imply certain distances from my ligand to its substrate. My goal is...

27 May 2024 7,082 1 View

Analytical frequency calculation error in ORCA 5.0.3, does anyone know how to change the calculation algorithm?

Good afternoon, We have recently started running ORCA calculation in a new machine, however we are consistently getting memory errors in analytical frequency calculations. Our jobs com-prise a...

27 March 2024 8,609 2 View

Which factors, practices, and tools impact trust in online business negotiations?

We addressed such a question at Exploring The Missing Link Between Trust in Business Negotia...

14 March 2024 2,875 1 View

LiF impurity on XRD data after HCl/LiF etching of Ti3C2 MXene ?

Hi, I've been etching Ti3AlC2 MAX phase in the lab for a year, having made several batches. In the last 3 I've been observing a peak at around 44°. This peak is observed for multilayer and...

11 March 2024 5,920 0 View

Can a medium containing 0.5% of FBS induce instant and drastic morphological changes in culture cells?

I'm doing a protocol in which I plate my cells in a 12-well plate with complete medium (i.e. DMEM/F-12 WITH 5% FBS), let them reach ~80% confluency and then I change the complete medium for a...

10 January 2024 8,232 5 View

Should I include non significant correlation coefficient while calculating summary effect size in random Meta analysis?

For calculating summary effect size in meta analysis do i need to group correlation/beta coefficient between 2 variables which are significant or even non significant ones. Eg I have 20 studies...

11 December 2023 1,532 2 View

How do I search a name in Zoological Record today?

Zoological Record today belongs to Clarivate and at their website, when I click at "Go to product" I am directed to Web of Science basic search. I did not find any filter for Zoological Record...

03 November 2023 8,752 1 View

How to exfoliate graphene in large areas (sides above 50 to 100 μm)?

I've been trying the method described in : Reliable Exfoliation of Large-Area High-Quality Flakes of Gr... The process I'm doing now is: Clean the SiO2/Si substrates with acetone, IPA and DI...

01 November 2023 9,236 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View