My colleague plans to do a hand pollination experiment on strawberry. This is going to be a quantitative experiment: we are trying to see how the quality of the pollens affects fruit set. Therefore, we need to pollinate each flower with the same amount of pollens. What's the best method to do this? Other technical advises about pollen collection and prevention of unwanted natural pollination would also be appreciated.
something that you should add to your study is examine the viability of your pollen. you can do it be placing pollen in drop of hanging solution of sugar and boron and count your germination and growth. If you will do your study in the greenhouse you will not need to paper bag the flowers.
That is exiting experiment. You need neuter the male parts of flowers in autogam plants.
It is suggested you plant the pollen of the plant in media contains sugar, agar and boron and count the germination percent in 3, 12 or even 24 hours times after plating pollens.
It is good idea that you test the pollen viability.
Good luck.
Determine pollen protein content and correlate with pollen viability Duy Minh Pham
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