In most grafting studies, I usually only see reports of the final survival rate (e.g. after 1 or 2 weeks 95% of scions survived). However, through personal experience with tomato, I observed that the graft could heal in largely different durations: some grafts could heal (i.e. the veins start to connect) after only 2 days, evidenced by the scion's leaves no longer withered, while others might not heal until day 6. And this difference significantly affected the graft plant's performance.
So I would like to ask if anyone knows a scientific method to assess whether the graft starts to heal (the veins successfully connect)? Assessment based on leaf withering as I mentioned above may not be very accurate. Some published references would be very helpful.
Madam/sir,
The final survival of grafts depends largely upon the method of grafting and the healing conditions. However the genotypic compatibility between the rootstock and scion plays a vital role in terms of graft survival as well as in determining the time taken to heal. To estimate the time taken for healing, anatomical observations on vascular regeneration can be taken up at different time intervals after grafting. This link (http://dx.doi.org/10.20546/ijcmas.2017.602.202) might be helpful.
Duy Minh Pham What is the successful rare for tomato grafting? In your experience?
Yuan-Yeu Yau
It varied from time to time, and depending on condition as well. I am trying to study how lighting affect graft healing. I don't use any humidifier, simply putting them in growth room with air conditioner, yet sometimes I could achieve up to 70-80% survival rate in all treatments. However, the time the grafts healed appeared to differ significantly among treatments. On day 2-3 only around 30-40% healed, but on day 6, 80% of them could be considered healed, or successfully grafted. But the later healed plants performed much worse (I continued to grow them up to 2 weeks after grafting), growth was stunned for a while and some parts of the leaves were withered beyond recovery. So I think this parameter is very important to assess.
Saheb Pal
Thank you for the suggestion. I've already thought of anatomical dissecting and microscopic observation. But this method would require a massive amount of samples and work to be able to evaluate quantitatively (we're looking at 10 samples/day x 3 replications for each treatment in 7 days, minimum). I am looking for a more direct method/parameter that we can use to assess the healing over time without having to kill the plants.
Replicate and go for anatomical dissection and microscopic observation of union of cambium layers
Please check these articles: Article Plant grafting: Insights into tissue regeneration
Article Evaluation of different grafting methods and rootstocks in w...
- Badges
- Science topic
- Similar topics
- Chemistry