How to find full length sequences of MYB genes of wheat?

Hi, How to find full length sequences of MYB genes of wheat?

08 September 2016 8,710 7 View

How to design gene specific primers?

06 July 2016 2,231 9 View

Is it RNA?

Hi, I extract RNA from leaf under normal & different stress condition, and synthesize cDNA but when I check its expression level with tubulin , untreated leaf give me normal result as show in...

05 June 2016 2,349 11 View

Gene expression under stress conditions?

Hi every one, I have a question that any body use PEG, NaCl and cold stresses to check gene expression under these stress conditions for checking expression level and what kind of tissues used e.g...

04 May 2016 4,724 11 View

Any one use QIAGEN RNase free DNase set (79254)? What about it results?

03 April 2016 5,397 5 View

Why my RNA always degrade?

Hi, I extract RNA from different plant tissues using Trizole as well as Qiagen RNAEasy kit but when ever i extract RNA by these two methods my RNA always degrade and have genomic DNA traces....

03 April 2016 4,515 4 View

How can I equalize RNA concentration of all samples?

03 April 2016 4,265 9 View

How to design primers?

02 March 2016 9,436 5 View

Which step is important in Primer designing?

Hi everybody, I want to know the critical steps in designing primers, means what will suitable Tm value, GC content, false priming, dimer, cross dimer, hairpin, rating, length of primer, product...

02 March 2016 6,727 3 View

Is degraded RNA converted to cDNA by RT PCR using reverse transcriptase?

02 March 2016 7,156 7 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View