How to find full length sequences of MYB genes of wheat?

Hi, How to find full length sequences of MYB genes of wheat?

08 September 2016 8,710 7 View

How to design gene specific primers?

06 July 2016 2,231 9 View

Is it RNA?

Hi, I extract RNA from leaf under normal & different stress condition, and synthesize cDNA but when I check its expression level with tubulin , untreated leaf give me normal result as show in...

05 June 2016 2,349 11 View

Gene expression under stress conditions?

Hi every one, I have a question that any body use PEG, NaCl and cold stresses to check gene expression under these stress conditions for checking expression level and what kind of tissues used e.g...

04 May 2016 4,724 11 View

Any one use QIAGEN RNase free DNase set (79254)? What about it results?

03 April 2016 5,397 5 View

Why my RNA always degrade?

Hi, I extract RNA from different plant tissues using Trizole as well as Qiagen RNAEasy kit but when ever i extract RNA by these two methods my RNA always degrade and have genomic DNA traces....

03 April 2016 4,515 4 View

How can I equalize RNA concentration of all samples?

03 April 2016 4,265 9 View

How to design primers?

02 March 2016 9,436 5 View

Which step is important in Primer designing?

Hi everybody, I want to know the critical steps in designing primers, means what will suitable Tm value, GC content, false priming, dimer, cross dimer, hairpin, rating, length of primer, product...

02 March 2016 6,727 3 View

Is degraded RNA converted to cDNA by RT PCR using reverse transcriptase?

02 March 2016 7,156 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View