Arijit Mukherjee

10 Questions 6 Answers 0 Followers

Questions related from Arijit Mukherjee

How to infect MS-agar grown Arabidopsis with Hyaloperonospora Arabidopsidis (Hpa)?

Hello Researchers, I am trying to infect Arabidopsis grown in MS-agar medium with Hpa pathogen (the downy mildew pathogen). However, it seems they can't infect well when plants grown in MS-agar...

24 April 2025 5,089 1 View

How can we quantify the number of each bacterial strain in a mix of two bacterial strains?

I wish to enumerate the number of each type of bacterial cells in a mix of two strains. How can I do that without tagging individual strains?

06 September 2024 9,565 5 View

Could anyone suggest an alternative gelling agent for sulfur deificent media gelation?

Dear researchers, I am trying to study S-deficiency in plants using a custom made agar media which is S-deficient. However, I am facing an issue when I changed my gelling agent from Phytagel to...

19 October 2023 3,766 0 View

How to design primers for T-DNA insertion lines from ABRC using iSect tool?

Hello Arabidopsis researchers, I was trying to use the iSect tool for designing primers of T-DNA insertion lines. However, when I am entering the stock number (all of them starts with CS), it...

09 June 2023 3,896 2 View

How to remove sulfate from agar?

Hi Researchers, I am working on sulfur deficiency in arabidopsis plants and facing a strange issue that the Arabidopsis plants are not showing any deficiency symptoms even if I remove sulfate...

05 June 2023 4,658 3 View

Growing arabidopsis under nutrient deficient media. No changes in phenotypes even under nutrient deficiency?

Dear Researchers it is quite surprising to me that suddenly the arabidopsis plants I'm growing under S-deficient (15uM sulfate) is not even showing deficiency phenotype when grown in Custom made...

25 May 2023 6,605 0 View

Root exudates collection for Arabidopsis using hydroponics system- How to remove MS salts?

Hi all , I am performing root exudates collection for Arabidopsis using Hydroponics based system. However, I am unable to find any protocol where people have colleceted exudates in liquid MS...

11 October 2022 3,913 4 View

TA cloning, insertion orientation of gene of interest, always forward?

Hello everyone, I am performing TA cloning (blunt end) for my gene of interest and while screening for the insertions, I always find my gene is inserted in a forward direction. Whereas by...

14 August 2022 6,189 5 View

High number of reads in blank controls for Amplicon sequencing data?

Hi Community, Recently I have submitted samples for amplicon and found an unusually high number of reads being present in my negative control blank samples. Yet, when I ordinated those samples in...

20 May 2022 2,312 0 View

16S amplicon for leaf endophytes using 799F-1391R primer pair, no bands?

Hello Researchers, I am performing leaf endophyte 16S amplicon from Arabidopsis plants grown in natural soil in growth chamber. I have collected the leaves (5 weeks), surface sterilised and...

03 June 2021 4,499 4 View