Hi all ,
I am performing root exudates collection for Arabidopsis using Hydroponics based system. However, I am unable to find any protocol where people have colleceted exudates in liquid MS media and performed LC-MS analysis based on that. Whereas most protocols say, they collect exudates in sterile MQ water. Can we perform root exudates analysis using the exudates collected in liquid MS in hydroponics system?
Appreciate any lead on this angle, thanks.
Ya that can be done. You have to run LCMS for MS basal, which will serve as a control. And then you proceed with your experiment. Compare both for eliminating media components. It looks simple but is rather difficult.
It may not be possible for you to remove MS salts as the roots also will absorb some of them.
Hi Arijit Mukherjee ,
You can dry the media, and/or do the extraction using polar or nonpolar solvent, depending on your expected compound's polarity.
Cheers,
Dharmesh
Hi Dharmesh Jain Swaroopa Ram Ghatge-Patil.
I dried the media, but the salt conc is too high and interferes with sample prep for LC/MS. I wish to know whether the media itself can be infused in LC/MS. Do the salts interfere with metabolites identification?
The salts will not interfere in the metabolites but may be used up by the roots as well. For LC/MS you can have a plain MS run to eliminate the media components.
And if you are working with the exudates, drying will not help you I think.
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