Hi Researchers,
I am working on sulfur deficiency in arabidopsis plants and facing a strange issue that the Arabidopsis plants are not showing any deficiency symptoms even if I remove sulfate from the media. I am growing them in a modified MS salt (without sulfur) with 0.8% agar. I learned that agar may contain sulfate and therefore some researchers do remove them. However, if any of you have any real experience of removing sulfate from agar, could you let me know in detail.
My growth condition:
1. Surface sterilize and vernalize for 3d in 4"C
2. Germinate in full strenght MS media for 7d
3. Transfer to S-sufficient or S-deficient media and then study the effect.
I have tested the salts components and seems there are no problem with either media or seed stocks. If you have any other suggestions to obtain the desired response from Arabidopsis plants, please let me know.
Your help is much appreciated.
Best
Arijit
You could enzymatically pretreat your agar to convert any sulphate into volatile H2S which would be driven off during autoclaving.
Also are you using ultrapure agar (like the sort used for gel electrophoresis)?
I assume you have substituted the ammonium sulfate, cupric sulfate, ferric sulfate, manganese sulfate, nickel sulfate, potassium sulfate, sodium sulfate, adenine hemisulfate and peptone in the media.
Hi Robert Adolf Brinzer
Thanks for your reply. Yes, the custom MS salt is devoid of any sulfur. For agar, now I'm trying out phytagel or gellan gum (from sigma). Do you have any idea that the phytagel does not contain any sulfur?
Only advice I can give is check the certificate of analysis for your batch.
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