I want to develop 4T1 tumor model. How many cells are needed to develop a subcutaneous tumor in Balb C mice?
Should I can use male Balb C mice in place of female?
If anyone have any clue about this, please guide me ASAP.
You should be careful about how many passage you did. Freshly expanded cells induce subcutaneous tumor at 1 to 5 x10ˆ5.
Male Balb/C is better. I am always avoiding the female hormones, if possible.
Good luck!!
Usually 10^5 cells are needed to develop a tumour.Smaller dose might work as well.You could select the right dose through trial and error.
Do the experiment to determine your tumor dose 100 (TD100), the minimal dose where all mice grown tumor. Our TD100 for 4T1 is around 1 x 10^3 cells in the mammary fat pad. So we use 5 x 10^3 cells for our experiments.
Cell lines can vary slightly, so it's best to find out what your own TD100 really is. Plus with a more minimal dose there is a better chance your therapy will work (if that's what you're testing).
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