24 Questions 41 Answers 0 Followers
Questions related from Anh Hoang Le
I want to use FAC to study integrins on the cell surface and I've heard some people claim that Trypsin can induce integrin degradation. I'm just wondering if anyone has seen any significant...
07 July 2019 5,019 3 View
Im working on a protein. We have our cell line and we suspect that the protein is present there. However, we don't have any commercial antibody for this protein so we cannot do any western blot to...
10 October 2018 3,826 4 View
It sounds like a quite straightforward question, however I find it's actually something very difficult to quantify. The problem is that cells have different thickness, and so when you take the...
04 April 2018 290 0 View
At the moment, I'm trying to analyse a difficult set of data that I've been thinking but really don't know how to do it. So basically, i have two populations of cells and I notice one of them...
02 February 2018 3,691 2 View
So I have made several stable melanoma cell lines expressing a protein of interest using retroviral vector. The cells survive antibiotic selection while those without the construct are all dead....
12 December 2017 670 4 View
So for a nested experimental design, you have subgroups: For example: 2 cell types: A and B and you do an IF A: 50 cells were randomly selected for analysis B: 50 cells were also chosen Now you...
09 September 2017 1,262 1 View
Does anybody know why GFP, including GFP-tagged proteins usually go into the nucleus apart from its normal cytoplasmic localisation? I checked NLS predictor but doesn't seem to suggest any NLS...
08 August 2017 7,689 2 View
Does anyone know any side effect of using this inhibitor in cell culture? I've been using this inhibitor on melanocytes but quite frequently I have observed very weird effects of this inhibitor...
05 May 2017 282 3 View
Perhaps this is a long standing question that bothers biologists, especially those at the early stage like me. So for example, I have a set of data with n = 3. Let say: Control:...
04 April 2017 1,850 29 View
For example, if you have 4 samples A, B, C, D: A = Control sample B, C, D are samples at different conditions. Assume all assumptions are met. Can you just simply use t-test Can you just simply...
04 April 2017 3,958 8 View
I'm analysing some imaging data but having a bit of a problem. When the images were taken, some of them have uneven shading. I want to set a threshold to analyse the area certain features within...
02 February 2017 1,003 6 View
I'm just wondering if anyone has any experience about the non-specific binding of GFP to the cytoskeleton, particularly actin fibers? Is it possible for the GFP protein to interact with actin...
02 February 2017 552 4 View
The question says it all. With normal PCR, since you don't have the methylated-DNA digestion step, you can simply stop after PCR or Restriction digest or ligation step. But with Q5 site directed...
01 January 2017 7,551 1 View
So I designed some primers but the melting temperature is all above 70 degree. I know that this should not be done, but to meet all the criteria, there is no other way. My main primer is 21 base...
12 December 2016 4,235 5 View
I'm having a problem with gelatin coating. My gelatin for some reason solidifies prematurely and when i look under the microscope, i can see a lot of lumps. I tried many different ways from...
12 December 2016 3,720 7 View
I have a tube of permeabilisation buffer (used for immunostaining) and Glycine 20mM solution. How long do you think I can store these at room temperature (basically keep these on my bench)?...
11 November 2016 7,245 4 View
I have some melanocytes from mouse that have been frozen down in liquid Nitrogen. Now I thawed out and reculture them, I dont know how many passages the cells have gone through before because...
11 November 2016 3,914 10 View
I'm having a question about equilibrium vs steady state.Is it true to say equilibrium is just a subset of steady state? Because what I think is for a steady state system, the rate change over time...
10 October 2015 9,585 1 View
My question is very simple, why in Laue crystallography, we use polychromatic X-rays? I've done some research on Google but the answer was not clear. There was one website mentioned very briefly...
10 October 2015 409 11 View
As we all know, with a linear chromosome, on the lagging strand (template 5'->3') of DNA replication, when the last piece of RNA primer at the 3' end is removed, the DNA cannot be extended and...
02 February 2015 7,663 11 View
I hope someone can clear this out for me. I find them a bit confusing and also a bit contradictory. Basically, we all know that Eo is the standard redox potential relative to the Standard Hydrogen...
02 February 2015 9,548 2 View
I'm having one question concerning the driving force for a mitochondrial Na+/H+ antiporter. This antiporter translocate 1 H+ into the matrix for 1 Na+ into the intermembrane space. The H+ can then...
02 February 2015 466 14 View
As everyone knows: Vmax = Kcat x [E]t with [E]t is total enzyme CONCENTRATION Vmax unit eg umol.min-1 (Im sorry, I mistyped this before) Kcat unit should be min-1 or s-1 So my question is, if...
01 January 2015 8,832 14 View
I guess this is an age old question but as academia is ever more competitive nowadays, as a young PhD student, I would like to ask those who have made it to their PI position what do they think is...
01 January 1970 1,699 2 View
