Does anyone know any side effect of using this inhibitor in cell culture?
I've been using this inhibitor on melanocytes but quite frequently I have observed very weird effects of this inhibitor on the behaviour (the results dont match the expected prediction which Im very confident with) of the cells. Do you know if it somehow affects the internal cytoskeleton of the cells? Or if this molecule can be taken up inside the cells if incubated for too long (overnight) and mock up the cell behaviour?
Do you know if it somehow affects the internal cytoskeleton of the cells? Or if this molecule can be taken up inside the cells if incubated for too long (overnight) and mocks up the cell behaviour? Even when you wash away the inhibitor after overnight incubation, the outcome afterward is still very weird and misbehaving.
Anyone who has experience, please share.
Thank you very much
Hello
See links here
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3367802/
https://www.ncbi.nlm.nih.gov/pubmed/21040999
http://onlinelibrary.wiley.com/doi/10.5966/sctm.2015-0194/full
Good Luck
its less likely that the effect you are observing is a direct intracellular than it is that it is effecting cell anchorage. It depends on your culture conditions i.e. if you are using FN-collagen coated vessels, etc. The other possibility is that it is interfering with normal growth factor metabolism, i.e. binding of growth factors to ECM.
Follow up with the question, I did another experiment where I incubate the cells with this molecule but did NOT wash it away but directly fix and stain it with phalloidin. From what I saw, the cells don't have their characteristic actin rich dots in them anymore. Usually my cells have a lot of these dots, but these dots just disappear in these GM6001 treated cells. It's really weird.
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