Amit Kumar Acharzo

12 Questions 10 Answers 0 Followers

Questions related from Amit Kumar Acharzo

Which quantitative LFA strip reader can provide precise test-line intensity measurement with minimal variability?

I am developing an LFA device and need to quantify test-line signal intensity reliably. Currently, I take photos of the strips and analyze grayscale intensity in ImageJ software. However, small...

last month 6,908 1 View

How to Reduce Non-Specific Test Line Signal in LFIA When Using Only Running Buffer?

Hi everyone, I’m currently developing a lateral flow immunoassay (LFIA) and encountering an issue with non-specific binding at the test line, even when applying only the running buffer (no...

24 June 2025 7,924 1 View

Why does PEG-3350 at 68 mg/mL induce a test line signal in lateral flow assays without antigen?

In our current project, we're working with the Rapid SARS-CoV-2 Antigen Test Card and have incorporated polyethylene glycol (PEG-3350) at a concentration of 68 mg/mL into the running buffer....

27 April 2025 7,653 2 View

How to calculate homogeneous chemical equilibrium constants from shifts in peak positions for ion transfer in immiscible electrolyte solutions?

i need a method to calculate the homogeneous chemical equilibrium constants from shifts in peak positions for ion transfer in immiscible electrolyte solutions

13 October 2023 6,795 0 View

How can I inhibit the Precipitation of Poorly Water-Soluble Drugs from syrup Formulations ?

I have developed a syrup at pH 3 of a BCS class II molecule using propylene glycol, beta Cyclodextrin, citric acid, antimicrobial preservative, and sweeting agent. But i every time get...

17 May 2022 8,536 3 View

Why don't I get tablet assay of more than 90% in my formulation?

I have developed a sustained release tablet of a BCS class III molecule (highly soluble in acidic media) with POLYOX™ WSR N60K and Polyethylene Glycol 6000. I am getting less than 90% in every...

17 May 2022 2,759 0 View

Why solute back from a solution when increasing pH?

I dissolved a poorly soluble drug molecule into a solution of 0.1N HCl and β-cyclodextrin, but when I increased the pH by adding NaOH, the solute returned from the solution and turned the solution...

18 January 2022 503 3 View

What are the scopes of mass spectrometry elucidation signalling hubs in cellular stress pathways to investigate cellular plasticity and adaptation?

I am looking for effective articles on the mass spectrometry elucidation signalling hubs in cellular stress pathways to investigate cellular plasticity and adaptation?

10 May 2021 2,794 1 View

Why does my formulated tablet crumbled into large fragments instead of tiny granules or particles during in vitro dissolution testing?

I am developing a tablet of bcs class 2 molecule. I used here Magnesium stearate, microcrystalline cellulose, povidone K-30, sodium starch glycolate. Coating: Hypromellose, iron oxide black, PEG...

01 May 2021 2,023 6 View

What is the example of hydrogel forming agent having molecular weight range between 100,000 and 8,000,000?

I am trying to develop of a Sustain release drug (BCS class I). so that, I want to use a hydrogel forming agent which molecular weight out of range between 100,000 and 8,000,000. I am also wanting...

27 March 2021 249 3 View

What are the impacts of pKa1and pKa2 in drug absorption ?

Pregabalin is a crystalline solid with a pKa1 of 4.2 and a pKa2 of 10.6. what insight can i get from this aforementioned information during drug design. What is the difference between pKa1and pKa2.

29 December 2020 7,472 3 View

What are the best references to guide me in choosing granulation method during preparation multivitamin tablet in combination with mineral ?

i am working on manufacturing Vitamin A, E, K and B complex tablet ( B1, B6, B2, B3 and B12 ) in combination with minerals but i confuse to choose granulation method ( direct compression and...

22 February 2020 7,796 3 View