Am I doing the mixed ANOVA right wnen there is many missed values?

01 February 2021 4,695 7 View

How to take into account the high resolution of the x-ray difractometer?

At small angles or in case high collimation, the peaks become anisotropic and with a Lorentz addition. The resolution describes correctly only with adding Lorentz and anisatropy parameters. It...

26 January 2021 5,829 4 View

Meshing a point could and exporting it into an *obj file format?

Anyone, any idea which software will better handle a point cloud to ---> triangular mesh generation with an option to export it into the *obj format? Thanks

01 April 2020 8,459 3 View

How many errors are there in SciFinder and other CAS databases?

14 February 2020 3,016 3 View

I need a rewiew of thermodynamic calculations of my preprint - who can help ?

I have a preprint here: Energy from humid air: reinventing the tornado I need to find the thermodynamics specialist to make a review of the calculations. It is about 600 lines of VBA code in MS...

26 March 2019 8,789 2 View

What metrics are using to compare detection methods?

Hello! In my researches I work with face detection compare methods (on Viola-Jones example). But I still have not found face detection metrics survey (or something like), or object detection...

21 June 2018 4,874 9 View

Dear colleagues, are there any opinions about the modern concept and prospects of development of "Noogenesis"?

05 June 2018 2,310 10 View

Why our universe is nearly flat?

At present, the geometry of the universe is only slightly different from the Euclidean model. This imposes very strong limitations on the curvature of the universe in the early stages of its...

11 April 2018 981 16 View

Are there automatic force field generators/services comparable in quality to OPLS?

What do you think about SwissParam, MolMeccano, GAAMP?

03 September 2017 1,499 4 View

No title

I believe that this species of subfamilies Epilachninae from Africa (Uganda)

08 January 2017 8,811 10 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

09 February 2021 3,779 6 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View