I am working on toxin detection by one protein and my protein is in immidazole solution. Can i use it for HPLC?
"imidazole" in solution (probably water ?).
BEFORE using the solution, you will dilute the solution into your HPLC mobile phase so the total amount (concentration) will be very small and should not pose any issues if good chromatography fundamentals are followed. *Please have an experienced chromatographer at your school assist you with the analysis to insure the best outcome.
Yes! There are many specialty HPLC columns that can tolerate an alkaline pH going up to 12. As I recall Imidazole has a pH of ~8.
The tiny volume of imidazole will be in the injection solution only so no concerns regarding mobile phase basic pH (or silica dissolving). *No LC method was provided.
Yes, you can use an imidazole solution for HPLC, but it is important to select the appropriate HPLC column and mobile phase that are compatible with your sample and the buffer used to prepare it.
Imidazole is a common buffer used in protein purification and analysis, and it is often used in HPLC methods for protein separation and characterization. However, the choice of HPLC column and mobile phase will depend on the specific properties of your protein and the toxin you are trying to detect.
For example, some proteins may require a reverse-phase column with a hydrophobic stationary phase and an organic mobile phase, while others may require a size-exclusion column with an aqueous mobile phase. Additionally, the pH and salt concentration of the buffer can also affect the separation and resolution of your sample.
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