How to quantifying Endosome and Dectin-1 internalization?

08 May 2018 0 2K Report

Hi Every one,

I am doing some IF experiments to see the early endosome after Dectin-1 activation by glucan. I am using Alexa 647 to tag Dectin-1 and EEA1 for endosome. I have two channels in confocal to microscopy them butt I do not know what is the best way to measure co-localization of Dectin-1 and Endosome in both channels or how to quantify these images.

Could you please help me if you have any experience?

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