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Questions related from Akila Wijerathna Yapa
I see MS Excel has several trend-line options; linear, logarithmic, polynomial, exponential, and power functions. What is the basis / logic of selecting these functions for biological data. For...
04 April 2018 2,764 3 View
Is there any significant difference between -20°C and -80°C stored biological samples (i.e. Plant tissues for proteins, mRNA and metabolite extraction)?
01 January 2018 1,650 3 View
Do plants have similar PI3K-Akt signaling pathway? What proteins involve?
10 October 2017 3,712 1 View
Can I use LB and RB boarder primers to amplify the either sides of the trans-gene (cassette)? For this does it necessary to check the copy number of the transgene first?
08 August 2017 6,051 3 View
If a same gene is clone either endogenous promoter or constitutive promoter (35S), Do we get a significantly different protein abundance?
08 August 2017 2,217 13 View
What are the differences between RNAi and artificial microRNA? is there any advantage of any over other type in creating mutants? How to design the cassette for RNAi and microRNA constructs for...
02 February 2017 9,286 0 View
Today molecular biology researchers allow generating massive quantities of multiple types of data with Nextgeneration sequencing, qPCR, microRNA, and Proteomics in less time. For an example, if...
01 January 2017 1,699 3 View
How TOR regulate the autophagy in plants (Arabidopsis)? Since tor mutants are embryo lethal, what mutants have been used so far for studying autophagy in plants?
01 January 2017 7,036 3 View
How to make a cDNA (pAD) library for Y2H protein-protein interaction. If possible let me know step by step with possible illustrations with practical aspects. What is the possibility of using...
08 August 2016 9,454 3 View
How over expression of receptor protein affects in vivo (plants)? (biochemically) using ectopic promoters / interact protein etc.....
07 July 2016 4,076 1 View
How to draw dot plot for different groups and denote comparisons ? (See attached images) If there is a procedure in SPSS/ Excel or if there is any free user friendly online S/W guide me. Thank you
06 June 2016 7,226 6 View
What may be the reason that ABA deficient mutants (aba2 / aba3) have high chlorophyll content comparing to WT. Any scientific paper available regarding this?
06 June 2016 4,643 3 View
What is the statistical tool to compare sample means reference to specific sample mean? How to use SPSS / Minitab S/W for this purpose? e.g. I want to compare the means of these samples with "Mu"...
06 June 2016 6,553 6 View
We are seeking to buy CAS 503-07-1 ( /-)-cis-12,13-Epoxy-9(Z)-octadecenoic / Vernonia oil (seed oil) for LC-MS. All the chemical companies that we've tried are not producing this at the...
05 May 2016 9,800 1 View
I've been , using spectrophotometer to measure chlorophyll content and following Equations were used (µg/ml or mg/l). These equations are for 1 cm path length. [Cha] =12.64 A664 - 2.99 A647[Chb]...
03 March 2016 1,536 3 View
What book would you recommend for life science research student to understand the concepts of statistics (parametric / non parametric) from the beginning? Also, practical approach with SPSS / Minitab?
02 February 2016 9,790 6 View
In every illustration and available resources it says that the two major pathways for repair of DSB is either homologous recombination (HR) or nonhomologous end joining (NHEJ) in crispr cas9...
12 December 2015 6,095 2 View
How to explain the difference and similarity application of statistical tool between regression and ANOVA.
01 January 2015 4,942 4 View
How same genetic makeup express differently? (some genes expressed only in certain types of cells, while not expressed in other types) If you can please attached notes / reading materiel (with...
12 December 2014 8,257 3 View
Does Km (Michaelis constant) vary with enzyme concentration Vmax depends on enzyme concentration since Vmax = 2Km why Km does not depend on enzyme concentration if Km is the substrate...
11 November 2014 5,520 16 View
In RNA extraction we are not expecting to break the nuclear envelope.So, there is no need to use a strong lysis solution. What is the main difference between DNA or RNA extraction? What should the...
11 November 2014 7,324 8 View
What are the freely available software / websites for this? For example, if I need to compare the six possible amino acid sequences for each polynucleotide sequences, how do I do it? Can anybody...
11 November 2014 7,470 2 View
Is there a way to translate amino acid sequences into nucleotide sequences? Are there any programs that can convert the amino acid sequences for the domains back into the corresponding nucleotide...
11 November 2014 5,049 4 View
What are the best methods to analyse nominal, ordinal, interval, and ratio data in Life Sciences / Agricultural Sciences with reference to Non-parametric & parametric tests? Do you've a...
08 August 2014 3,245 8 View
What are good resources for learning mathematics and statistics for life science with applied examples with basic knowledge? Are there any free books with easy guide.
08 August 2014 5,479 3 View
How can I generate the equation of a curve that matches all arbitrarily given (x,y) pairs? How do I find the closest matching curve? I expect it to be something like : y = ax2 ± bx ± c I'd be much...
08 August 2014 2,093 7 View
Can someone explain differential screening of subtractive cDNA library with illustration?
08 August 2014 742 2 View
What are the very crucial steps in control of plant gene expression and what is the importance of timing (mRNA transport control, mRNA translation control, mRNA & protein stability &...
08 August 2014 10,022 3 View
How do they figure out in diagrams? What are the meaning of Arrows usually denote the orientation of repetitive sequences in figures. what is the important of that? What are the function of repeat...
08 August 2014 3,621 5 View
How same sequence once read from one direction to make one protein and in the opposite direction to make a different one? How is the function occur and what will be the crucial point? timing of...
08 August 2014 3,893 3 View
What are the good study material for learning the application of mathematics for biology?
07 July 2014 4,215 3 View
Why there are two kinds of Logarithm? Where to use them separately ? common logarithm vs natural logarithm? When solving a problem such as 5x = 25, will I get the same answer when I use common...
07 July 2014 8,261 3 View
After designing my own primers, how can I confirm and generate their desired location on sequence is perfect (Primer map)? Can someone suggest any other free and easy software available...
02 February 2014 2,520 11 View
I'm working on CCCVd molecular detection work. Expected RNA present very low concentration in samples ( ≤ 1 μL per sample). When following nested PCR, at first PCR unable to get the expected size...
01 January 2014 9,418 1 View
What are the conditions that should be taken to isolate Cccvd Viroid by Polyacrylamide gel electrophoresis? (** CCCVd Viroids are small, single-stranded RNA molecules, ranging from 246 to 375...
12 December 2013 789 0 View
After statistical analysis, how can i show the significant differences (S.D) in bar plots? In SPSS 16 and Minitab 15 there is no such function ( to show any symbol above the bar). I've analysed...
10 October 2013 3,474 9 View
A good method to identify a gene in any living organism is by the other living organism which is close to the first in taxonomy and then make all the molecular work to find out if that gene does...
10 October 2013 8,907 3 View
need your help to find a solution for my problem to indicate significant differences in a bar chart plot. The data of the statistical test is available in the following...
12 December 2012 7,995 37 View
20 % polyacrylamide gel solution was poured in to the gel assembly and comb was inserted. Gel was allowed to polymerize in at least 2 hours and assembly was pre-run in 1X TBE at 200 V for 60...
11 November 2012 3,573 2 View
I've used 4M Guanidine Hydro Chloride (GuHCl) for Plant RNA Viroid extraction with 25mM EDTA, 0.2M Sodium Acetate (NaAc) (pH 5), 2.5% PVP 40. Could you please tell me ; How RNA get separated from...
11 November 2012 4,051 10 View
