Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

Books on doing a PhD?

Hi colleagues, I wondered if any of you could recommend a book for a student starting out on her PhD. Her subject area is: health, education, training and child...

07 December 2020 549 4 View

Is fluoro-jade staining possible with tissue-cleared brains?

I am hoping to stain whole mouse brain hemispheres with fluoro-jade C as a marker of degeneration. We are presently working to optimize this staining for tissue cleared with the LifeCanvas SHIELD...

10 March 2020 9,709 1 View

Antibacterial liquid inhibition assay (MIC) with LB medium?

Is it possible to determine the antibacterial minimum inhibitory concentration (MIC) using a liquid inhibition assay (MIC) with Lysogeny broth medium instead of Mueller-Hinton broth? If so, is...

09 February 2020 6,330 5 View

Does it help for a prospective graduate student to have a research proposal in hand when applying to grad school?

This coming semester, I will be teaching an undergraduate class on graduate school preparation for science majors. One of the main assignments during the class will be the assembly of a full...

17 December 2019 8,239 11 View

Looking for validated questionnaires to use with both children and parents on reporting Emotional Health/difficulties?

Hi there, I'm currently doing my MSc in Psychology of Education. I'm looking to answer the following research questions for my dissertation? a) Are there differences in self-reporting of...

13 December 2019 8,779 5 View

Can a cell membrane be overloaded with too many particles attempting to enter?

When more than one biomolecules attempt to enter into a cell membrane at once (in a large quantity) does it impede the transfection process?

23 July 2019 4,149 2 View

How do I improve agarose gel electrophoresis to determine alleles (c.100bp)?

I'm trying to determine parentage based upon microsatellite markers, and using agarose gel electrophoresis to visualise the different lengths. Fragment size is c.100bp and having trouble getting...

01 February 2018 7,171 3 View

How to improve DNA yields using DNA extraction kits designed for soil or sediment samples?

02 November 2017 9,029 3 View

How do you respond to requests for article reprints for articles which were published in journal to which you assigned copyright?

If the publication journal 'owns' the article and the request is not for teaching/educational purposes from someone who is not a current collaborator, do such requests fall under 'fair use' or...

17 October 2017 5,325 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View