I have performed the primary cell cultures from wild-type Danio rerio blastomeres using DMEM (medium). The results of cultures observed with inverted microscopy showed not very good results. Here are some related questions about it.
1. What might cause the cell is not attached (adherent)? Is contamination capable of causing non adherent cells?
2. What do you think about the images I have included? Can the contamination be determined from bacteria or certain organism?
3. What are the obstacles to cells to reproduce/proliferate themselves?
4. What are the factors influence the cell signaling and interfere division and differentiation?
5. Is it bad if the incubation was done in 28 degrees of Celcius?