Dear RG community,
We have a X. fastidiosa strain in the lab acquired from DSMZ (DSM 103417) and it is being really hard to grow it in the lab.
We are not able to achieve the growth rates described in the literature when using the same media.
We have been using several growth media and gelling agents (BCYE, PW, GYE, agar, gel-rite).
Can you share your insight on how to deal with this culture?
Thank you so much for your help,
BR
Carla Pinheiro
did you tried the DMSZ 1052 medium ?
https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1052.pdf
Xylella fastidiosa is a plant pathogenic bacterium, not typically cultured for general purposes. However, if necessary, grow it in a sterile culture medium containing essential nutrients, maintain a pH around 7, and incubate at 28°C. Use appropriate safety precautions due to its potential impact on plants.
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