Why my deltaATG mRNA is in polysome fractions ?

Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...

16 December 2020 7,622 2 View

HowScholarships, Fellowships and grants in Materials engineering for international students to study abroad?

On Line student doctorant looking for first Master PhD student doctorant as UNICAF now in Materials engineering for international students to study abroad on Line and To Bricks LTGS and To have...

10 December 2020 3,686 3 View

Merging two same papers in a one paper?

Hello, I have three same papers which have different numbers of citations and different number of readings and I would like to merge these three papers in one paper with taking account of a new...

20 September 2020 5,275 6 View

Does polysome profiling in 4mL tube instead of 12 mL needs a scale down in ultracentrifugation time ?

Hello everyone, I recently started several polysome profiling experiment and something bugs me out. Every protocols I found use 12mL tube. They load their lysate containing ribosomes attached to...

19 September 2020 8,717 10 View

What is the sensitivity of SHM technique based on electromechanical impedance method to identify a crack in a concrete beam?

The sensitivity of the technique is important to know how to identify the appearance of cracks

17 August 2020 4,156 9 View

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31 May 2020 6,077 6 View

How can you efficiently collect a ground-nesting bee when it is inside its nest?

I am looking for an efficient way to collect a ground-nesting bee at its nest, so that when encountering a ground-nesting bee nest, I could determine what species created that nest. Ideally, I was...

21 February 2020 5,269 3 View

Does someone has an interest for XIXth century herbaria prepared in Brazil?

While investigating about French women botanists in the XIXth Century I chanced upon two herbarias, made from plants collected in Brazil. I am looking for someone who could help to evaluate the...

07 October 2019 2,560 6 View

Journals to submit a method to assess Vulnerability of Hotels to Earthquakes ?

Dear all, What are the most appropriate journals to submit a method we developed to estimate the seismic vulnerability index for touristic accommodations ? and its application to a pilot zone of...

08 September 2019 8,878 3 View

The "4p1000" initiative on soil carbon storage: Should the name be changed?

At the COP 21, in France, a few years back, an initiative was proposed to increase the carbon content of soils by 0.4% or 4 per 1000 per year. The claim was made that, in so doing, soils could...

28 May 2019 3,080 12 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View