Add zinc acetate or zinc chloride 1mM and incubate 5 min at 37C (room temp also work  but 37 C gives best precipitation) hemoglobin will precipitate specifically. Do not use too much zinc that will precipitate other proteins (mainly HSA ) also.

Add zinc acetate or zinc chloride 1mM and incubate 5 min at 37C (room temp also work  but 37 C gives best precipitation) hemoglobin will precipitate specifically. Do not use too much zinc that will precipitate other proteins (mainly HSA ) also.

Hi Punith,

the following paper might also be an option

Erythrocyte lysis in isotonic solution of

ammonium chloride: Theoretical modeling and

experimental verification

ARTICLE in JOURNAL OF THEORETICAL BIOLOGY · APRIL 2008

best regards

I am facing the same problem Punith

I'm reading some papers to find out what I can do.

1) Depletion of hemoglobin and carbonic anhydrase from erythrocyte cytosolic samples by preparative clear native electrophoresis (Nature Protocols 7, 36–44

(2012) doi:10.1038/nprot.2011.427 Published online 08 December 2011)

2) An easy preparative gel electrophoretic method for targeted depletion of hemoglobin in erythrocyte cytosolic samples (Volume 32, Issue 11, pages 1319–1322, June 2011)

3) Separation of human erythrocyte membrane associated proteins with one-dimensional and two-dimensional gel electrophoresis followed by identification with matrix-assisted laser desorption/ionization-time of flight mass spectrometry.

4) Proteome analysis of the triton-insoluble erythrocyte membrane skeleton

5) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications

6) ALTERATION OF ALPHA-SPECTRIN UBIQUITINATION FOLLOWING HEMORRHAGIC SHOCK

Kindly follow the attached paper.