Rafael Factor Carandina

5 Questions 11 Answers 0 Followers

Questions related from Rafael Factor Carandina

Characterization of exosomes?

I have started to work with isolation of exosomes and I used the protocol from this paper (ultracentrifuge): "Isolation and characterization of exosomes from cell culture supernatants and...

06 June 2018 8,978 2 View

What is the minimum amount of exosomes to enrich a culture medium?

I want to enrich a culture medium with isolated exosomes of a given cell line for cell signaling studies in another kind of cells. What is the minimum amount of exosomes to enrich this culture medium?

05 May 2018 2,741 3 View

Is FBS sufficient for papain inactivation? Why/Why not? Do I really need to use Ovomucoid in my Papain Stop Solution?

I am trying to dissociate cerebellar neurons and I don't have Ovomucoid or E-64.

04 April 2017 5,861 3 View

(Western Blotting/ SDS PAGE / Proteomics) should the sample preparation for studying membrane proteins be different for preparing cytosolic proteins?

I'm trying to find out which membrane protein (~ 30kDa) my samples of mesenchymal stem cells are expressing. I made a SDS-PAGE and the gel was stained with Coomassie Blue. The band in question is...

06 June 2016 9,365 3 View

Does anyone here work with intestinal cell primary cultures?

Intestinal epithelial organoids

08 August 2013 8,135 11 View